Stoll P, Schwer CI, Goebel U, Buerkle H, Hoetzel A, Schmidt R. Hepatic steatosis prevents heme oxygenase-1 induction by isoflurane in the rat liver. World J Gastroenterol 2011; 17(37): 4184-4190 [PMID: 22072849 DOI: 10.3748/wjg.v17.i37.4184]
Corresponding Author of This Article
Rene Schmidt, MD, DESA, Department of Anesthesiology and Critical Care Medicine, Freiburg University Medical Center, Hugstetter Strasse 55, D-79106 Freiburg, Germany. rene.schmidt@uniklinik-freiburg.de
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Original Article
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World J Gastroenterol. Oct 7, 2011; 17(37): 4184-4190 Published online Oct 7, 2011. doi: 10.3748/wjg.v17.i37.4184
Hepatic steatosis prevents heme oxygenase-1 induction by isoflurane in the rat liver
Patrick Stoll, Christian I Schwer, Ulrich Goebel, Hartmut Buerkle, Alexander Hoetzel, Rene Schmidt
Patrick Stoll, Christian I Schwer, Ulrich Goebel, Hartmut Buerkle, Alexander Hoetzel, Rene Schmidt, Department of Anesthesiology and Critical Care Medicine, Freiburg University Medical Center, D-79106 Freiburg, Germany
Author contributions: Stoll P performed all animal experiments and most of the molecular analyzes and wrote the first draft of the manuscript; Schwer CI participated in the research; Goebel U participated in data analysis; Buerkle H wrote the paper; Hoetzel A participated in research design and data analysis; Schmidt R designed the study and wrote the paper.
Supported by Departmental funding
Correspondence to: Rene Schmidt, MD, DESA, Department of Anesthesiology and Critical Care Medicine, Freiburg University Medical Center, Hugstetter Strasse 55, D-79106 Freiburg, Germany. rene.schmidt@uniklinik-freiburg.de
Telephone: +49-761-27023060 Fax: +49-761-27023960
Received: December 30, 2010 Revised: April 7, 2011 Accepted: April 14, 2011 Published online: October 7, 2011
Abstract
AIM: To characterize the inductive effects of isoflurane (ISO) on hepatic heme oxygenase-1 (HO-1) in an animal model of hepatic steatosis.
METHODS: Lean (LEAN) and obese (FAT) Zucker rats were randomized into 4 groups: 1: LEAN + pentobarbital sodium (PEN); 2: LEAN + ISO; 3: FAT + PEN; 4: FAT + ISO. The animals were mechanically ventilated for 6 h. In vitro analyses of liver tissue included determination of HO-1 mRNA and protein expression as well as measurement of HO enzyme activity and immunohistochemical analyses.
RESULTS: Compared to PEN treatment, ISO administration profoundly induced hepatic HO-1 mRNA and protein expression and significantly increased HO enzyme activity in lean Zucker rats. In contrast, no difference in HO-1 gene expression was observed after ISO or PEN anesthesia in obese Zucker rats.
CONCLUSION: The present study demonstrates that ISO is an inducer of hepatic HO-1 gene expression in non-steatotic organs but failed to upregulate HO-1 in steatotic livers.
