Brief Article
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World J Gastroenterol. Jan 21, 2011; 17(3): 349-353
Published online Jan 21, 2011. doi: 10.3748/wjg.v17.i3.349
Detection of Helicobacter pylori: A faster urease test can save resources
Andriani Koumi, Theodoros Filippidis, Vassilia Leontara, Loukia Makri, Marios Zenon Panos
Andriani Koumi, Marios Zenon Panos, Department of Gastroenterology, Euroclinic of Athens, Athanassiadou 9, 11521 Athens, Greece
Andriani Koumi, Department of Gastroenterology, NIMTS Hospital, 11521 Athens, Greece
Theodoros Filippidis, Vassilia Leontara, Department of Histopathology, Euroclinic of Athens, Athanassiadou 9, 11521 Athens, Greece
Loukia Makri, Department of Statistics, Ministry of Finance, 1439 Nicosia, Cyprus
Author contributions: Koumi A and Panos MZ designed and performed the study and wrote the paper; Filippidis T and Leontara V performed the histology/immunohistochemistry; Makri L performed the statistical analyses.
Correspondence to: Dr. Marios Zenon Panos, MD, FRCP, AGAF, Department of Gastroenterology, Euroclinic of Athens, Athanassiadou 9, 11521 Athens, Greece. mpanos@euroclinic.gr
Telephone: +30-21-6416772 Fax: +30-21-6416530
Received: June 18, 2010
Revised: August 8, 2010
Accepted: August 15, 2010
Published online: January 21, 2011
Abstract

AIM: To investigate whether differences in the rapidity of a positive result for Helicobacter pylori can save resources, by comparing two commercially available urease kits.

METHODS: One hundred and eighty-five adults (130 outpatients, 55 inpatients) undergoing gastroscopy were entered prospectively. Patients were divided into two groups: Group 1 (if they were not on PPIs, antibiotics, H2A, bismuth or sucralfate for up to 14 d prior to the endoscopy) and Group 2 (if they were on, or had been on, any of the above medication in the previous 14 d). At endoscopy two sets of biopsies, taken in random order, were placed in the wells of the Campylobacter-like organism (CLO) test (Kimberly-Clark, Utah, USA) and the Quick test (Biohit Plc, Helsinki, Finland). Five additional gastric biopsies were taken for histology/Giemsa and immunohistochemical study. The two urease test slides were read at 2 min, 30 min, 2 h and 24 h. Sensitivity and specificity at 24 h were determined.

RESULTS: At 24 h, for all patients, there was no difference in sensitivity (100% vs 97.5%), specificity (99.3%), positive (97.5%) and negative predictive values (100% vs 99.3%) between the CLO and Quick tests, respectively. There was a positive result at 30 min in 17/41 (41.5%) CLO tests, and in 28/40 (70%) Quick tests, P = 0.05. Quick test enabled the prescription of eradication therapy before discharge in all 28/40 patients. Only 12 (30%) follow-up appointments were needed. If the CLO test had been used alone, only 17 (41.5%) prescriptions would have been possible prior to discharge and 24 (58%) follow-up appointments would be needed (P = 0.001). Of 2000 gastroscopies performed annually at our unit, a saving of 123 follow-up appointments (total: 8856 Euros or 11 808 USD) would be achieved if we switched to the Quick test.

CONCLUSION: Direct comparison of locally available urease test kits is worthwhile, since the appropriate choice results in a significant saving of resources. Local costs and follow-up protocols will determine the magnitude of these savings.

Keywords: Campylobacter-like organism test; Diagnosis; Helicobacter pylori; Quick test; Urease test kits