Huang C, Yang G, Jiang T, Cao J, Huang KJ, Qiu ZJ. Down-regulation of STAT3 expression by vector-based small interfering RNA inhibits pancreatic cancer growth. World J Gastroenterol 2011; 17(25): 2992-3001 [PMID: 21799645 DOI: 10.3748/wjg.v17.i25.2992]
Corresponding Author of This Article
Zheng-Jun Qiu, Professor, MD, Department of General Surgery, Affiliated First People’s Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai 200080, China. qiuwryb@online.sh.cn
Article-Type of This Article
Original Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Jul 7, 2011; 17(25): 2992-3001 Published online Jul 7, 2011. doi: 10.3748/wjg.v17.i25.2992
Down-regulation of STAT3 expression by vector-based small interfering RNA inhibits pancreatic cancer growth
Chen Huang, Guang Yang, Tao Jiang, Jun Cao, Ke-Jian Huang, Zheng-Jun Qiu
Chen Huang, Tao Jiang, Jun Cao, Ke-Jian Huang, Zheng-Jun Qiu, Department of General Surgery, Affiliated First People’s Hospital, Shanghai Jiao Tong University, Shanghai 200080, China
Chen Huang, Zheng-Jun Qiu, Shanghai Key Laboratory of Pancreas Disease, Shanghai 200080, China
Chen Huang, Zheng-Jun Qiu, Pancreatic Cancer Center of Shanghai Jiaotong University, Shanghai 200080, China
Guang Yang, Department of General Surgery, Taian Central Hospital, Taian 271000, Shandong Province, China
Author contributions: Huang C, Yang G and Jiang T contributed equally to this work; Huang C, Yang G, Jiang T and Qiu ZJ designed the experiment and conducted all laboratory work; Cao J and Huang KJ participated in the design and coordination of the work; Huang C, Yang G and Qiu ZJ drafted the manuscript; all authors have read and approved the final manuscript.
Supported by A Grant from the Science and Technology Commission of Shanghai Municipality, No. 09QA1404600; and a grant from the Affiliated First People’s Hospital of Shanghai Jiao Tong University, No. 0801
Correspondence to: Zheng-Jun Qiu, Professor, MD, Department of General Surgery, Affiliated First People’s Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai 200080, China. qiuwryb@online.sh.cn
Telephone: +86-21-63240090 Fax: +86-21-63240825
Received: December 16, 2010 Revised: February 21, 2011 Accepted: February 28, 2011 Published online: July 7, 2011
Abstract
AIM: To evaluate the effect of RNA interference (RNAi) mediated silence of signal transduction and activation of transcription (STAT)3 on the growth of human pancreatic cancer cells both in vitro and in vivo.
METHODS: STAT3 specific shRNA was used to silence the expression of STAT3 in pancreatic cancer cell line SW1990. The anti-growth effects of RNAi against STAT3 were studied in vitro and in experimental cancer xenografts in nude mice. The potential pathways involved in STAT3 signaling were detected using reverse transcription polymerase chain reaction and western blotting.
RESULTS: The expression of the STAT3 was inhibited using RNAi in SW1990 cells. RNAi against STAT3 inhibited cell proliferation, induced cell apoptosis and significantly reduced the levels of CyclinD1 and Bcl-xL when compared with parental and control vector-transfected cells. In vivo experiments showed that RNAi against STAT3 inhibited the tumorigenicity of SW1990 cells and significantly suppressed tumor growth when it was directly injected into tumors.
CONCLUSION: STAT3 signaling pathway plays an important role in the progression of pancreatic cancer, and silence of STAT3 gene using RNAi technique may be a novel therapeutic option for treatment of pancreatic cancer.
