Brief Article
Copyright ©2011 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. May 28, 2011; 17(20): 2572-2579
Published online May 28, 2011. doi: 10.3748/wjg.v17.i20.2572
Differential protein expression during colonic adaptation in ultra-short bowel rats
Hai-Ping Jiang, Tao Chen, Guang-Rong Yan, Dan Chen
Hai-Ping Jiang, Tao Chen, Dan Chen, Department of General Surgery, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China
Guang-Rong Yan, Institute of Life and Health Engineering and National Engineering and Research Center for Genetic Medicine, Jinan University, Guangzhou 510632, Guangdong Province, China
Author contributions: Jiang HP and Chen T contributed to the study design and data interpretation; Jiang HP, Chen T and Yan GR performed the majority of experiments and data analysis; Chen D was responsible for the quality control of the formula; Chen T contributed to the quality control of animals; Jiang HP, Chen T and Yan GR wrote the manuscript.
Supported by A Grant from the Natural Science Foundation of Guangdong Province, China, No. 07005961
Correspondence to: Guang-Rong Yan, PhD, Associate Professor, Institute of Life and Health Engineering and National Engineering and Research Center for Genetic Medicine, Jinan University, Guangzhou 510632, Guangdong Province, China. tgryan@jnu.edu.cn
Telephone: +86-20-85224372 Fax: +86-20-38688306
Received: December 31, 2010
Revised: April 13, 2011
Accepted: April 20, 2011
Published online: May 28, 2011
Abstract

AIM: To investigate the proteins involved in colonic adaptation and molecular mechanisms of colonic adaptation in rats with ultra-short bowel syndrome (USBS).

METHODS: Sprague Dawley rats were randomly assigned to three groups: USBS group (10 rats) undergoing an approximately 90%-95% small bowel resection; sham-operation group (10 rats) undergoing small bowel transaction and anastomosis; and control group (ten normal rats). Colon morphology and differential protein expression was analyzed after rats were given post-surgical enteral nutrition for 21 d. Protein expression in the colonic mucosa was analyzed by two-dimensional electrophoresis (2-DE) in all groups. Differential protein spots were detected by ImageMaster 2D Platinum software and were further analyzed with matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight-mass spectrometric (MALDI-TOF/TOF-MS) analysis.

RESULTS: The colonic mucosal thickness significantly increased in the USBS group compared with the control group (302.1 ± 16.9 μm vs 273.7 ± 16.0 μm, P < 0.05). There was no statistically significant difference between the sham-operation group and control group (P > 0.05). The height of colon plica markedly improved in USBS group compared with the control group (998.4 ± 81.2 μm vs 883.4 ± 39.0 μm, P < 0.05). There was no statistically significant difference between the sham-operation and control groups (P > 0.05). A total of 141 differential protein spots were found in the USBS group. Forty-nine of these spots were down-regulated while 92 protein spots were up-regulated by over 2-folds. There were 133 differential protein spots in USBS group. Thirty of these spots were down-regulated and 103 were up-regulated. There were 47 common differential protein spots among the three groups, including 17 down-regulated protein spots and 30 up-regulated spots. Among 47 differential spots, eight up-regulated proteins were identified by MALDI-TOF/TOF-MS. These proteins were previously reported to be involved in sugar and fat metabolism, protein synthesis and oxidation reduction, which are associated with colonic adaption.

CONCLUSION: Eight proteins found in this study play important roles in colonic compensation and are associated with sugar and fat metabolism, protein synthesis, and molecular chaperoning

Keywords: Ultra-short bowel syndrome; Enteral nutrition; Colon adaptation; Proteomics