Original Article
Copyright ©2011 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. May 28, 2011; 17(20): 2563-2571
Published online May 28, 2011. doi: 10.3748/wjg.v17.i20.2563
siRNA targeting Livin decreases tumor in a xenograft model for colon cancer
Bo-Young Oh, Ryung-Ah Lee, Kwang Ho Kim
Bo-Young Oh, Ryung-Ah Lee, Kwang Ho Kim, Department of Surgery, Ewha Womans University School of Medicine, Seoul 158-710, South Korea
Author contributions: Oh BY performed statistical analysis and drafted the manuscript; Lee RA planned the investigation, supervised the laboratory work, helped with data analysis, helped to draft the manuscript, and revised the manuscript; Kim KH participated in the study design and coordination.
Supported by the Korea Research Foundation Grant #2007-E00037 funded by Korea government (MOEHRD, Basic Research Promotion Fund)
Correspondence to: Ryung-Ah Lee, MD, PhD, Department of Surgery, Ewha Womans University School of Medicine, Seoul 158-710, South Korea. ralee@ewha.ac.kr
Telephone: +82-2-26502659 Fax: +82-2-26447984
Received: October 6, 2010
Revised: February 15, 2011
Accepted: February 22, 2011
Published online: May 28, 2011
Abstract

AIM: To evaluate the effect of silencing Livin gene expression with siRNA to apoptosis and proliferation in a colon cancer cell line.

METHODS: To investigate the anticancer effect of silencing Livin gene expression, we established an siRNA transfected cell line using the HCT116 colon cancer cell line. After confirming the successful transfection, MTT assay, flow cytometry and annexin V staining were employed to evaluate the antiapoptotic effect. To confirm the in vivo effect of Livin-siRNA, different doses of Livin-siRNA were injected into xenografted tumors in BALB/c nude mice model.

RESULTS: Livin expression was dramatically decreased after siRNA transfection, especially at 25 μmol/L of siRNA, but this suppression was not dose-dependent. The cell count at 18 h after transfection was significantly reduced as compared with controls (P < 0.01), but tended not to decrease proportionally depending on transfected dose or time. MTT assay revealed that silencing the Livin gene suppressed cellular proliferation at 18 h after transfection (P = 0.04); however, the inhibitory effect disappeared thereafter. Also, there was no significant difference in cellular proliferation depending on siRNA dose. The rate of apoptosis also increased with silencing of the Livin gene. In vivo, the tumor size significantly decreased after Livin-siRNA injection at 20 μmol/L concentration (P = 0.03). There were no significant body weight changes of mice after siRNA injection. Histologic examination revealed no significant toxic reaction in kidney, liver and brain of mice.

CONCLUSION: siRNA-mediated downregulation of Livin expression can induce apoptosis in colon cancer in vitro and in vivo, which suggests the possibility of new cancer therapeutics using siRNA.

Keywords: siRNA; Livin; Inhibitor of apoptosis; Colon cancer