Brief Article
Copyright ©2011 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. May 21, 2011; 17(19): 2417-2423
Published online May 21, 2011. doi: 10.3748/wjg.v17.i19.2417
p53 antibodies, metallothioneins, and oxidative stress markers in chronic ulcerative colitis with dysplasia
Hala E Hamouda, Soha S Zakaria, Saber A Ismail, Mahmoud A Khedr, Wael W Mayah
Hala E Hamouda, Soha S Zakaria, Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, 31111, Egypt
Saber A Ismail, Mahmoud A Khedr, Wael W Mayah, Department of Tropical Medicine, Faculty of Medicine, Tanta University, Tanta, 31111, Egypt
Author contributions: Hamouda HE and Zakaria SS contributed equally to this work; Hamouda HE and Zakaria SS designed the study, performed the research, wrote the paper, analyzed the data and revised the paper; Ismail SA, Khedr MA and Mayah WW designed the research, diagnosed the patients, collected samples and revised the paper.
Correspondence to: Dr. Hala E Hamouda, Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Hamouda Ghoraba Street, Elstaad, Tanta, 31111, Egypt. hala_el-said@hotmail.com
Telephone: +20-40-3417454 Fax: +20-40-3337402
Received: January 10, 2011
Revised: February 6, 2011
Accepted: February 13, 2011
Published online: May 21, 2011
Abstract

AIM: To investigate the role of p53 antibodies (p53Abs), metallothioneins (MTs) and oxidative stress markers in the early detection of dysplasia in chronic ulcerative colitis (UC).

METHODS: The study included 30 UC patients, 15 without dysplasia (group II) and 15 with dysplasia (group III), in addition to 15 healthy volunteers (group I, control subjects). The enzyme-linked immunosorbent assay technique was used to measure serum p53Abs and MTs, while advanced oxidation protein products (AOPPs), and reduced glutathione (GSH) levels were measured by spectrophotometric method in all subjects.

RESULTS: In group II and group III compared to group I, there were significant increases in serum levels of AOPPs (145.94 ± 29.86 μmol/L and 192.21 ± 46.71 μmol/L vs 128.95 ± 3.06 μmol/L, P < 0.002 and P < 0.001, respectively), MTs (8.18 ± 0.35 μg/mL and 9.20 ± 0.58 μg/mL vs 6.12 ± 0.25 μg/mL, P < 0.05 and P < 0.05, respectively), and p53Abs (20.19 ± 3.20 U/mL and 34.66 ± 1.34 U/mL vs 9.42 ± 1.64 U/mL, P < 0.001 and P < 0.001, respectively). There were significantly higher levels of AOPPs (P < 0.05) and p53Abs (P < 0.001) in UC patients with dysplasia compared to those without dysplasia, while MTs showed no significant difference between the 2 groups (P > 0.096). In contrast, GSH levels showed a significant decrease in both patients’ groups (1.87 ± 0.02 μmol/mL and 1.37 ± 0.09 μmol/mL vs 2.49 ± 0.10 μmol/mL, P < 0.05 and P < 0.05 in groups II and III, respectively) compared with group I, and the levels were significantly lower in group III than group II (P < 0.05). There was a positive correlation between AOPPs and both MTs (r = 0.678, P < 0.001) and p53Abs (r = 0.547, P < 0.001), and also between p53Abs and MTs (r = 0.739, P < 0.001). There was a negative correlation between AOPPs and GSH (r = -0.385, P < 0.001), and also between GSH and both MTs (r = -0.662, P < 0.001) and p53Abs (r = -0.923, P < 0.001).

CONCLUSION: Oxidative stress and oxidative cellular damage play an important role in the pathogenesis of chronic UC and the associated carcinogenetic process. p53Abs levels could help in early detection of dysplasia in these conditions.

Keywords: Ulcerative colitis, Advanced oxidation protein products, Reduced glutathione, Metallothionein