Yan SY, Hu Y, Fan JG, Tao GQ, Lu YM, Cai X, Yu BH, Du YQ. Clinicopathologic significance of HER-2/neu protein expression and gene amplification in gastric carcinoma. World J Gastroenterol 2011; 17(11): 1501-1506 [PMID: 21472111 DOI: 10.3748/wjg.v17.i11.1501]
Corresponding Author of This Article
Shi-Yan Yan, MD, Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. shyyan2008phd@yahoo.com
Article-Type of This Article
Brief Article
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Shi-Yan Yan, Ying Hu, Jian-Gao Fan, Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
Guo-Quan Tao, Department of General Surgery, Huaian No.1 People's Hospital affiliated to Nanjing Medical University, Huaian 223300, Jiangsu Province, China
Yong-Ming Lu, Xu Cai, Bao-Hua Yu, Department of Pathology, Cancer Hospital, Fudan University, Shanghai 200032, China
Yi-Qun Du, Department of Pathology, Institute of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China
Author contributions: Yan SY participated in the treatment of patients, analyzed the data, performed the research and wrote the manuscript; Hu Y and Fan JG participated in the treatment of patients, collected the data and designed the research; Tao GQ, Du YQ and Cai X collected all the cancer samples and Clinicopathologic data of gastric carcinoma patients; Other authors contributed to the new reagents/analytic tools.
Supported by National Natural Science Foundation of China, No. 81071888
Correspondence to: Shi-Yan Yan, MD, Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. shyyan2008phd@yahoo.com
Telephone: +86-21-25078999 Fax: +86-21-25078999
Received: August 8, 2010 Revised: December 13, 2010 Accepted: December 20, 2010 Published online: March 21, 2011
Abstract
AIM: To study the HER-2/neu protein expression and gene amplification in gastric carcinoma and their relation.
METHODS: One hundred and forty-five formalin-fixed and paraffin- embedded tumor tissue samples from Chinese gastric carcinoma patients were studied with immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) methods. Clinicopathologic data about all patients were collected.
RESULTS: The levels of HER-2 3+, HER-2 2+ and HER2 1+ were measurable in 6.9%, 8.3% and 17.2% of the samples, respectively. No HER-2 was stained in 67.6% of the samples. FISH showed that HER-2 gene was amplified in 18 samples, 10 HER-2 3+ samples, 5 HER-2 2+ samples, and 3 HER-2 1+ samples with IHC staining. HER-2 status was not correlated with the sex and age of patients, and tumor size, location or differentiation, but with the depth of invasion, TNM stage, lymph node and distant metastasis as well as histopathological classification of gastric cancer (P < 0.05).
CONCLUSION: All samples with IHC as HER-2 expression should be analyzed with FISH. Detection of HER-2 gene amplification can assess the malignant biological behaviors and prognosis of gastric cancer.
