Li Y, Li B, Zhang Y, Xiang CP, Li YY, Wu XL. Serial observations on an orthotopic gastric cancer model constructed using improved implantation technique. World J Gastroenterol 2011; 17(11): 1442-1447 [PMID: 21472102 DOI: 10.3748/wjg.v17.i11.1442]
Corresponding Author of This Article
Xiao-Ling Wu, Professor, Department of Gastroenterology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China. xiaolianbei@sina.com
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Original Article
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World J Gastroenterol. Mar 21, 2011; 17(11): 1442-1447 Published online Mar 21, 2011. doi: 10.3748/wjg.v17.i11.1442
Serial observations on an orthotopic gastric cancer model constructed using improved implantation technique
Yan Li, Bo Li, Yu Zhang, Chun-Ping Xiang, Yuan-Yuan Li, Xiao-Ling Wu
Yan Li, Xiao-Ling Wu, Department of Gastroenterology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
Bo Li, Department of Surgery, the First People’s Hospital, Jiangbei District, Chongqing 400020, China
Yu Zhang, Department of Pathology, the First People’s Hospital, Jiangbei District, Chongqing 400020, China
Chun-Ping Xiang, Department of Pathology, Children’s Hospital, Chongqing Medical University, Chongqing 400014, China
Yuan-Yuan Li, Department of Pathology, Chongqing Medical University, Chongqing 400016, China
Author contributions: Li Y conceived of the study, constructed animal models, and drafted the manuscript; Li B participated and established the animal model; Zhang Y and Xiang CP performed paraffin sections and hematoxylin and eosin staining; Li YY performed immunohistochemical staining; Wu XL guided and inspected the whole experiments.
Supported by the Natural Science Foundation of China, No. 30830040
Correspondence to: Xiao-Ling Wu, Professor, Department of Gastroenterology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China. xiaolianbei@sina.com
Telephone: +86-23-63693325 Fax: +86-23-63711527
Received: October 12, 2010 Revised: December 30, 2010 Accepted: January 6, 2011 Published online: March 21, 2011
Abstract
AIM: To establish a gastric cancer nude-mouse model with improved orthotopic implantation and investigate its biological characteristics at different time points.
METHODS: Human gastric cancer SGC-7901 cell suspensions were injected subcutaneously into a nude mouse to develop solid tumors, and the tumor tissue pieces were implanted under the serous coat. The nude mice were then euthanized in group every two weeks to observe the primary tumor growth and metastases.
RESULTS: Within 2-4 wk, there were no obvious changes about the primary tumor in stomach. At the sixth week, the primary tumor began to grow fast, resulting in incrassation of the gastric wall and stenosis of the gastric cavity, and metastases into the liver and lymph nodes were detected. The tumor, which compressed the adjacent organs, gradually became bigger and bigger followed by stenosis or vanishment of the gastric cavity from 8 to 12 wk. There were massive metastases, and the rate of metastasis was 58% in lymph nodes, 78% in liver, 39% in kidney, and 81% in peritoneum or septum.
CONCLUSION: A gastric cancer model is established, which can simulate the clinical tumor behavior and provide experimental carrier for clinical trials of gastric cancer treatment.
