Original Article
Copyright ©2010 Baishideng. All rights reserved
World J Gastroenterol. Mar 7, 2010; 16(9): 1063-1069
Published online Mar 7, 2010. doi: 10.3748/wjg.v16.i9.1063
Factors influencing intercellular spaces in the rat esophageal epithelium
Dong-Hong Zhang, Li-Ya Zhou, Xiu-Yun Dong, Rong-Li Cui, Yan Xue, San-Ren Lin
Dong-Hong Zhang, Li-Ya Zhou, Xiu-Yun Dong, Rong-Li Cui, Yan Xue, San-Ren Lin, Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, China
Author contributions: Zhou LY, Zhang DH, Lin SR and Dong XY designed the study; Zhang DH, Dong XY and Cui RL performed the majority of the experiments; Zhang DH, Zhou LY and Dong XY analyzed the data; Zhang DH wrote the manuscript; Zhou LY, Lin SR and Xue Y revised the manuscript.
Correspondence to: Dr. Li-Ya Zhou, Department of Gastroenterology, Peking University Third Hospital, No. 49 Huayuanbei Road, Beijing 100191, China. liyazhou@medmail.com.cn
Telephone: +86-10-82266699 Fax: +86-10-62034716
Received: November 7, 2009
Revised: December 29, 2009
Accepted: January 6, 2010
Published online: March 7, 2010
Abstract

AIM: To evaluate the effect of acute stress, hydrochloric acid, ethanol, aspirin, and prednisolone on the intercellular spaces of the esophageal epithelium.

METHODS: Part I, male Sprague-Dawley rats were randomly divided into eight groups and treated with the damaging or control factors. The esophagus of each rat was macroscopically inspected. Histological changes in mucosal biopsies were examined by light microscopy, and the widths of intercellular spaces were determined by transmission electron microscopy (TEM). Part II, in part I, we found that acute stress and aspirin induced dilated intercellular spaces (DIS) of the esophageal epithelium. Therefore, the effect of acid suppression pretreatment with esomeprazole on esophageal epithelial DIS induced by water immersion and restraint stress (WRS) and aspirin was further investigated to determine the association of DIS with acid reflux. After administration of 0.9% sodium chloride solution or esomeprazole solution orally for five days, rats underwent WRS or intragastric administration of aspirin solution. Esophageal epithelial intercellular spaces were investigated by TEM.

RESULTS: (1) The five damaging factors produced no lesions or inflammation in esophageal mucosa of rats under either gross or routine histological inspections. Esophageal epithelial intercellular space diameters in stress and aspirin groups were significantly greater, nearly three or two-fold respectively, than those in their corresponding control groups (stress model: 0.38 ± 0.05 μm vs 0.13 ± 0.02 μm, P < 0.01; aspirin model: 0.32 ± 0.12 μm vs 0.19 ± 0.05 μm, P < 0.01). Neither intragastric administration of hydrochloric acid or ethanol, nor hypodermic injection of prednisolone produced DIS compared with their corresponding control groups (hydrochloric acid model: 0.24 ± 0.03 μm vs 0.19 ± 0.05 μm, P > 0.05; ethanol model: 0.25 ± 0.10 μm vs 0.19 ± 0.05 μm, P > 0.05; prednisolone model: 0.20 ± 0.03 μm vs 0.14 ± 0.03 μm, P > 0.05); and (2) No significant difference in the intercellular space diameters was observed between the group pretreated with esomeprazole and the control group, in both the stress and aspirin models (stress model: 0.35 ± 0.05 μm vs 0.37 ± 0.05 μm, P > 0.05; aspirin model: 0.24 ± 0.02 μm vs 0.27 ± 0.03 μm, P > 0.05).

CONCLUSION: Acute stress and aspirin can induce DIS of the esophageal epithelium in rats, and it is not correlated with acid reflux.

Keywords: Esophagus, Dilated intercellular spaces, Stress, acute, Aspirin, Reflux, acid