Brief Article
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World J Gastroenterol. Feb 7, 2010; 16(5): 603-607
Published online Feb 7, 2010. doi: 10.3748/wjg.v16.i5.603
Metabolic investigations in patients with hepatitis B and C
Abdul Aziz Mastoi, Bikha Ram Devrajani, Syed Zulfiquar Ali Shah, Qasim Rohopoto, Sikander Ali Memon, Marya Baloch, Ghulam Ali Qureshi, Waqas Sami
Abdul Aziz Mastoi, Sikander Ali Memon, Marya Baloch, M.A.Kazi Institute of Chemistry, University of Sind, Jamshoro 75000, Pakistan
Bikha Ram Devrajani, Syed Zulfiquar Ali Shah, Qasim Rohopoto, Department of Medicine, Liaquat University of Medical and Health Sciences, Jamshoro 75000, Pakistan
Ghulam Ali Qureshi, Medical Research Centre, Liaquat University of Medical and Health Sciences, Jamshoro 75000, Pakistan
Waqas Sami, Department of Biochemistry, University of Health Sciences, Lahore 54600, Pakistan
Author contributions: Devrajani BR, Mastoi AA and Rohopoto Q designed the study, collected, analyzed and interpreted the data, drafted and revised the paper; Qureshi GA guided and assisted during the study period regarding the specific protocol; Memon SA, Sami W and Baloch M participated in collection of data; Shah SZA participated in drafting, data analysis and interpretation.
Correspondence to: Dr. Bikha Ram Devrajani, Department of Medicine, Liaquat University of Medical and Health Sciences, Jamshoro 75000, Pakistan. devrajani62@msn.com
Telephone: +92-22-2787999 Fax: +92-22-2729655
Received: October 19, 2009
Revised: November 25, 2009
Accepted: December 2, 2009
Published online: February 7, 2010
Abstract

AIM: To investigate the similarities and dissimilarities in patients with hepatitis B and hepatitis C, clinically and metabolically.

METHODS: Fifty patients with hepatitis B virus and hepatitis C virus infection were included in this study, along with fifty healthy controls for comparison purposes. Intravenous blood (10 mL) samples from patients and healthy subjects were collected and made to clot before serum was separated and immediately levels of the enzymes, alkaline phosphatase (ALK), creatinine phosphokinase (CPK), lactate dehydrogenase (LDH), serum glutamate oxaloacetate transaminase (s-GOT) and serum glutamate pyruvate transaminase (s-GPT) were determined by a kit method. For total content of each metal the serum samples were analyzed using atomic absorption spectrophotometry. Levels of cholesterol, triglycerides, urea, creatinine and uric acid were determined using a kit method on Microlab 300.

RESULTS: Serum magnesium and copper levels remained unchanged, whereas the concentration of zinc decreased and iron increased significantly in both groups of patients. Total antioxidant activity was significantly decreased in both hepatitis B and C. Among the enzymes analyzed, ALK, s-GPT, LDH and s-GOT were all significantly increased in both patients with hepatitis B and C whereas CPK was significantly decreased in patients with hepatitis B and remained unchanged in patients with hepatitis C.

CONCLUSION: The information accumulated by this study will help provide a better understanding of involved metabolic processes in order to design appropriate therapeutic approaches for treating these patients, so they can recover and lead normal lives.

Keywords: Hepatitis B virus; Hepatitis C virus; Antioxidant activity; Metal content; Enzyme activity