Brief Article
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World J Gastroenterol. Dec 28, 2010; 16(48): 6155-6162
Published online Dec 28, 2010. doi: 10.3748/wjg.v16.i48.6155
Increasing the frequency of CIK cells adoptive immunotherapy may decrease risk of death in gastric cancer patients
Jing-Ting Jiang, Yue-Ping Shen, Chang-Ping Wu, Yi-Bei Zhu, Wen-Xiang Wei, Lu-Jun Chen, Xiao Zheng, Jing Sun, Bin-Feng Lu, Xue-Guang Zhang
Jing-Ting Jiang, Chang-Ping Wu, Lu-Jun Chen, Xiao Zheng, Department of Tumor Biological Treatment, Third Affiliated Hospital of Soochow University, Changzhou 213003, Jiangsu Province, China
Jing-Ting Jiang, Yi-Bei Zhu, Jing Sun, Xue-Guang Zhang, Key Laboratory of Stem Cell of Jiangsu Province, Institute of Biotechnology, Key Laboratory of Clinical Immunology of Jiangsu Province, Soochow University, Suzhou 215123, Jiangsu Province, China
Yue-Ping Shen, Wen-Xiang Wei, Department of Medicine, Soochow University, Suzhou 215123, Jiangsu Province, China
Bin-Feng Lu, Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, United States
Author contributions: Jiang JT and Shen YP contributed equally to this work, designed the study and drafted the manuscript; Wu CP and Zhu YB participated in the experimental design; Wei WX and Chen LJ provided the clinical samples; Zheng X and Sun J analyzed the data and formatted the figures; Zhu YB performed all the experiments; Lu BF and Zhang XG drafted the manuscript and designed the study; all authors read and approved the final manuscript.
Supported by The National Natural Science Foundation of China, No. 30872176, 30950022 and 30972703; grants of Jiangsu Province and Soochow University Medical Development Foundation, No. EE126765
Correspondence to: Xue-Guang Zhang, MD, PhD, Key Laboratory of Stem Cell of Jiangsu Province, Institute of Biotechnology, Key Laboratory of Clinical Immunology of Jiangsu Province, Soochow University, Suzhou 215123, Jiangsu Province, China. xueguangzh@yahoo.com.cn
Telephone: +86-512-65125022 Fax: +86-512-65104908
Received: October 12, 2010
Revised: November 16, 2010
Accepted: November 23, 2010
Published online: December 28, 2010
Abstract

AIM: To analyze the correlation between cytokine-induced killer (CIK) cells adoptive immunotherapy and cancer-related death in gastric cancer patients.

METHODS: One hundred and fifty-six gastric cancer patients after operation at the Third Affiliated Hospital of Soochow University were enrolled in this study. Their clinical data including demographic characteristics, operation time, tumor size, pathological type and staging, tumor metastasis, outcome of chemotherapy or CIK cells adoptive immunotherapy, survival time or time of death were collected with a standard structured questionnaire. Kaplan-Meier method was used to estimate the median survival time, and the 2- and 5- year survival rates. Hazard risk (HR) and 95% confidence interval (95% CI) of CIK cells adoptive immunotherapy for gastric cancer were calculated using the two-stage time-dependent covariates Cox model.

RESULTS: The survival time of gastric cancer patients was longer after CIK cells adoptive immunotherapy than after chemotherapy (χ2 = 10.907, P = 0.001). The median survival time of gastric cancer patients was also longer after CIK cells adoptive immunotherapy than after chemotherapy (49 mo vs 27 mo, P < 0.05). The 2- and 5-year survival rates of gastric cancer patients were significantly higher after CIK cells adoptive immunotherapy than after chemotherapy (73.5% vs 52.6%, 40.4% vs 23.9%, P < 0.05). A significant difference was observed in the survival curve for patients who received CIK cells adoptive immunotherapy (0, 1-10, 11-25, and over 25 frequencies) (χ2 = 14.534, P = 0.002). The frequencies of CIK cells adoptive immunotherapy were significantly related with the decreasing risk of death in gastric cancer patients after adjustment for sex and age of the patients, tumor stage and relapse (HR = 0.54, 95% CI: 0.36-0.80) when the first stage Cox model was used to define the subjects who remained alive beyond 36 mo as survivors. However, no correlation was observed between the frequencies of death in CIK cells adoptive immunotherapy and the risk of gastric cancer patients (HR = 1.09, 95% CI: 0.63-0.89) when the second stage Cox model was used to define the subjects who survived for more than 36 mo as survivors.

CONCLUSION: The survival time of the gastric cancer patients treated with chemotherapy combined with CIK cells adoptive immunotherapy is significantly longer than that of the patients treated with chemotherapy alone and increasing the frequency of CIK cells adoptive immunotherapy seems to benefit patients more.

Keywords: Immunotherapy; Cytokine-induced killer cells; Gastric cancer; Survival analysis; Probability