Márquez L, Pérez-Nievas BG, Gárate I, García-Bueno B, Madrigal JL, Menchén L, Garrido G, Leza JC. Anti-inflammatory effects of Mangifera indica L. extract in a model of colitis. World J Gastroenterol 2010; 16(39): 4922-4931 [PMID: 20954278 DOI: 10.3748/wjg.v16.i39.4922]
Corresponding Author of This Article
Dr. Juan C Leza, Department of Pharmacology, School of Medicine, University Complutense and Centro de Investigación en Red de Salud Mental (CIBERSAM), 28040 Madrid, Spain. jcleza@med.ucm.es
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World J Gastroenterol. Oct 21, 2010; 16(39): 4922-4931 Published online Oct 21, 2010. doi: 10.3748/wjg.v16.i39.4922
Anti-inflammatory effects of Mangifera indica L. extract in a model of colitis
Lucía Márquez, Beatriz G Pérez-Nievas, Icíar Gárate, Borja García-Bueno, José LM Madrigal, Luis Menchén, Gabino Garrido, Juan C Leza
Lucía Márquez, Beatriz G Pérez-Nievas, Icíar Gárate, Borja García-Bueno, José LM Madrigal, Luis Menchén, Juan C Leza, Department of Pharmacology, School of Medicine, University Complutense and Centro de Investigación en Red de Salud Mental (CIBERSAM), 28040 Madrid, Spain
Lucía Márquez, Pharmaceutical Chemistry Center and University of La Habana, 11600 La Habana, Cuba
Beatriz G Pérez-Nievas, Icíar Gárate, Borja García-Bueno, José LM Madrigal, Juan C Leza, Health Research Institute, Hospital 12 de Octubre (I+12), 28040 Madrid, Spain
Luis Menchén, Gastroenterology Service, Hospital General Universitario Gregorio Marañón and CIBEREHD, 28040 Madrid, Spain
Gabino Garrido, Department of Chemistry and Pharmacy, University Católica del Norte, Antofagasta, 1270709, Chile
Author contributions: Márquez L carried out the experimental studies (animal treatments included) and drafted the manuscript; Pérez-Nievas BG and Gárate I did part of the biochemical work; Garrido G and Menchén L corrected the manuscript; García-Bueno B and Madrigal JLM performed blindly the statistical analysis; Leza JC designed the study and coordinated the final version of the manuscript; all authors read and approved the final manuscript.
Supported by Spanish Ministry of Education (MEC, SAF07-63138), the Instituto de Salud Carlos III, Mental Health Research Network, CIBERSAM, and Foundation Santander-UCM (GR 58/08)
Correspondence to: Dr. Juan C Leza, Department of Pharmacology, School of Medicine, University Complutense and Centro de Investigación en Red de Salud Mental (CIBERSAM), 28040 Madrid, Spain. jcleza@med.ucm.es
Telephone: +34-91-3941478 Fax: +34-91-3941464
Received: May 10, 2010 Revised: May 28, 2010 Accepted: June 5, 2010 Published online: October 21, 2010
Abstract
AIM: To investigate the effect of aqueous extract from Mangifera indica L. (MIE) on dextran sulfate sodium (DSS)-induced colitis in rats.
METHODS: MIE (150 mg/kg) was administered in two different protocols: (1) rectally, over 7 d at the same time as DSS administration; and (2) once daily over 14 d (by oral gavage, 7 d before starting DSS, and rectally for 7 d during DSS administration). General observations of clinical signs were performed. Anti-inflammatory activity of MIE was assessed by myeloperoxidase (MPO) activity. Colonic lipid peroxidation was determined by measuring the levels of thiobarbituric acid reactive substances (TBARS). Reduced glutathione (GSH) levels, expression of inflammatory related mediators [inducible isoforms of nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, respectively] and cytokines [tumor necrosis factor (TNF)-α and TNF receptors 1 and 2] in colonic tissue were also assessed. Interleukin (IL)-6 and TNF-α serum levels were also measured.
RESULTS: The results demonstrated that MIE has anti-inflammatory properties by improvement of clinical signs, reduction of ulceration and reduced MPO activity when administered before DSS. In addition, administration of MIE for 14 d resulted in an increase in GSH and reduction of TBARS levels and iNOS, COX-2, TNF-α and TNF R-2 expression in colonic tissue, and a decrease in IL-6 and TNF-α serum levels.
CONCLUSION: MIE has anti-inflammatory activity in a DSS-induced rat colitis model and preventive administration (prior to DSS) seems to be a more effective protocol.
