Paeonol inhibits tumor growth in gastric cancer in vitro and in vivo

doi:10.3748/wjg.v16.i35.4483

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Sep 21, 2010 (publication date) through Nov 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 21, 2010; 16(35): 4483-4490
Published online Sep 21, 2010. doi: 10.3748/wjg.v16.i35.4483

Paeonol inhibits tumor growth in gastric cancer in vitro and in vivo

Na Li, Lu-Lu Fan, Guo-Ping Sun, Xin-An Wan, Zhang-Gui Wang, Qiang Wu, Hua Wang

Na Li, Lu-Lu Fan, Guo-Ping Sun, Xin-An Wan, Zhang-Gui Wang, Department of Oncology, the First Affiliated Hospital of Anhui Medical University, Hefei 230032, Anhui Province, China

Qiang Wu, Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China

Hua Wang, Department of Oncology, Anhui Provincial Hospital, Hefei 230032, Anhui Province, China

Author contributions: Li N and Fan LL performed the majority of experiments and wrote the manuscript; Sun GP, Wang H and Wu Q designed the study and were involved in revising the manuscript; Wan XA and Wang ZG provided vital reagents and analytical tools.

Supported by Grants from National Natural Science Foundation of China, No. 30772537; and Natural Science Foundation of Anhui Province, No. 00044414 and No. 050430901

Correspondence to: Dr. Guo-Ping Sun, Department of Oncology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China. sunguoping@ahmu.edu.cn

Telephone: +86-551-2922354 Fax: +86-551-5161208

Received: April 6, 2010
Revised: June 4, 2010
Accepted: June 11, 2010
Published online: September 21, 2010

Abstract

AIM: To investigate the anti-tumor effects of paeonol in gastric cancer cell proliferation and apoptosis in vitro and in vivo.

METHODS: Murine gastric cancer cell line mouse forestomach carcinoma (MFC) or human gastric cancer cell line SGC-7901 was cultured in the presence or absence of paeonol. Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell cycle and apoptosis by flow cytometry and TUNEL staining. Tumor growth after subcutaneous implantation of MFC cells in mice was monitored, and the effects of treatment with paeonol were determined.

RESULTS: In vitro, paeonol caused dose-dependent inhibition on cell proliferation and induced apoptosis. Cell cycle analysis revealed a decreased proportion of cells in G0/G1 phase, with arrest at S. Paeonol treatment in gastric cancer cell line MFC and SGC-790 cells significantly reduced the expression of Bcl-2 and increased the expression of Bax in a concentration-related manner. Administration of paeonol to MFC tumor-bearing mice significantly lowered the tumor growth and caused tumor regression.

CONCLUSION: Paeonol has significantly growth-inhibitory and apoptosis-inducing effects in gastric cancer cells both in vitro and in vivo.

Keywords: Paeonol; Gastric cancer; Cell proliferation; Apoptosis