Huang TC, Tsai SS, Liu LF, Liu YL, Liu HJ, Chuang KP. Effect of Arctium lappa L. in the dextran sulfate sodium colitis mouse model. World J Gastroenterol 2010; 16(33): 4193-4199 [PMID: 20806438 DOI: 10.3748/wjg.v16.i33.4193]
Corresponding Author of This Article
Kuo Pin Chuang, PhD, Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 912, Pingtung, Taiwan, China. kpchuang@mail.npust.edu.tw
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World J Gastroenterol. Sep 7, 2010; 16(33): 4193-4199 Published online Sep 7, 2010. doi: 10.3748/wjg.v16.i33.4193
Effect of Arctium lappa L. in the dextran sulfate sodium colitis mouse model
Tzou-Chi Huang, Shinn-Shyong Tsai, Li-Fang Liu, Yu Lin Liu, Hung-Jen Liu, Kuo Pin Chuang
Tzou-Chi Huang, Department of Food Science, National Pingtung University of Science and Technology, 912, Pingtung, Taiwan, China
Shinn-Shyong Tsai, Department of Veterinary Medicine, National Pingtung University of Science and Technology, 912, Pingtung, Taiwan, China
Li-Fang Liu, Department of Life Science, National Pingtung University of Science and Technology, 912, Pingtung, Taiwan, China
Yu Lin Liu, Hung-Jen Liu, Kuo Pin Chuang, Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 912, Pingtung, Taiwan, China
Hung-Jen Liu, Institute of Molecular Biology, National Chung Hsing University, 250, Kuo Kung Rd., Taichung, Taiwan, China
Author contributions: Huang TC and Chuang KP designed the research, acquisition and analysis of data; Tsai SS and Liu HJ revise the research critically for important intellectual content; Liu LF and Liu YL performed part of the research; Chuang KP wrote the paper.
Supported by Grants to Kuo Pin Chuang from the National Science Council (NSC), No. NSC-98-2313-B-020-009; and Council of Agriculture, No. 98-9.2.1-B1(5)
Correspondence to: Kuo Pin Chuang, PhD, Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 912, Pingtung, Taiwan, China. kpchuang@mail.npust.edu.tw
Telephone: +886-8-7703202 Fax: +886-8-7700447
Received: May 25, 2010 Revised: June 8, 2010 Accepted: June 15, 2010 Published online: September 7, 2010
Abstract
AIM: To analyze the possible protective role of Arctium lappa L. (AL) in a murine model of ulcerative colitis (UC).
METHODS: BALB/c mice were administered 100 mg/kg AL powder orally each day. After 7 d, colitis was induced by administration of dextran sulfate sodium (DSS) (5% W/V) in drinking water for a further 8 consecutive days. Diarrhea and bloody stools as well as colonic histology were observed. The level of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in colonic sections were detected by immunohistochemistry.
RESULTS: There were significant differences in mean body weight values and disease activity indices between controls and AL-treated animals. Moreover, the histological findings showed that AL treatment can prevent mucosal edema, submucosal erosions, ulceration, inflammatory cell infiltration and colon damage. In addition, immunohistochemistry analysis showed that the levels of the inflammatory cytokines, IL-6 and TNF-α were also decreased in AL-treated groups.
CONCLUSION: We suggest that AL can prevent intestinal damage and decrease inflammatory cytokines in mice with DSS-induced colitis. Thus, AL could prove to be a useful food for UC.
