Mota LG, Coelho LG, Simal CJ, Ferrari ML, Toledo C, Martin-Comin J, Diniz SO, Cardoso VN. Leukocyte-technetium-99m uptake in Crohn’s disease: Does it show subclinical disease? World J Gastroenterol 2010; 16(3): 365-371 [PMID: 20082484 DOI: 10.3748/wjg.v16.i3.365]
Corresponding Author of This Article
Valbert N Cardoso, PhD, Radioisotope Laboratory, Pharmacy Faculty, Federal University of Minas Gerais, Avenue Antônio Carlos 6627, 31270-901, Belo Horizonte, Minas Gerais, Brazil. cardosov@farmacia.ufmg.br
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World J Gastroenterol. Jan 21, 2010; 16(3): 365-371 Published online Jan 21, 2010. doi: 10.3748/wjg.v16.i3.365
Leukocyte-technetium-99m uptake in Crohn’s disease: Does it show subclinical disease?
Luciene G Mota, Luiz GV Coelho, Carlos JR Simal, Maria LA Ferrari, Clodomiro Toledo, Josep Martin-Comin, Simone OF Diniz, Valbert N Cardoso
Luciene G Mota, Nuclear Engineering Department, Federal University of Minas Gerais, 31270-901, Belo Horizonte, Brazil
Luiz GV Coelho, Maria LA Ferrari, Clodomiro Toledo, Gastroenterology ALFA Institute, Clinical Hospital, Federal University of Minas Gerais, 31270-901, Belo Horizonte, Brazil
Carlos JR Simal, Nuclear Medicine, Felício Rocho Hospital, Medicine Faculty, Federal University of Minas Gerais, 31270-901, Belo Horizonte, Brazil
Josep Martin-Comin, Medicine Nuclear Center, Bellvitge Hospital, 08907, Barcelona, Spain
Simone OF Diniz, Valbert N Cardoso, Radioisotope Laboratory, Pharmacy Faculty, Federal University of Minas Gerais, 31270-901, Belo Horizonte, Brazil
Author contributions: Mota LG, Cardoso VN, Diniz SOF and Simal CJR were responsible for radiolabeling and scintigraphic images; Coelho LGV, Ferrari MLA and Toledo C contributed to the selection of patients; Martin-Comin J provided the initial idea for this study.
Supported by Fundação de Amparo a Pesquisa do Estado de Minas Gerais and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Correspondence to: Valbert N Cardoso, PhD, Radioisotope Laboratory, Pharmacy Faculty, Federal University of Minas Gerais, Avenue Antônio Carlos 6627, 31270-901, Belo Horizonte, Minas Gerais, Brazil. cardosov@farmacia.ufmg.br
Telephone: +55-31-34096892 Fax: +55-31-34096985
Received: September 4, 2009 Revised: October 13, 2009 Accepted: October 20, 2009 Published online: January 21, 2010
Abstract
AIM: To evaluate inflammatory activity in patients with Crohn’s disease (CD) using technetium-99m-hexamethylpropyleneamine oxime (99mTc-HMPAO) granulocyte scintigraphy.
METHODS: Twenty patients (7 male and 13 female) with CD and five healthy volunteers were selected for 99mTc-HMPAO granulocyte scintigraphy. The Crohn’s Disease Activity Index (CDAI), blood tests and C-reactive protein (CRP) of each patient were performed 7 d before the scintigraphic images. The leukocytes were labeled according to the International Society of Radiolabeled Blood Elements (ISORBE) consensus protocol and the scintigraphic images, including single photon emission computed tomography, were obtained 30 min and 2 h after injection of the radiolabeled leukocytes.
RESULTS: The labeling yield of the leukocytes with the lipophilic complex 99mTc-HMPAO was 55.0% ± 10%. Six of the 20 patients (30%) presented congruent results for the three parameters investigated (CDAI, Scintigraphic Index and CRP). On the other hand, 14 patients (70%) did not show congruent results. There was no significant correlation between the indices analyzed according to the Spearman test (P > 0.05, n = 20).
CONCLUSION: The results suggest that 99mTc-HMPAO-labeled leukocyte scintigraphy could be important for determining inflammatory activity in CD even in the absence of clinical symptoms.
