Antibiotics and probiotics in chronic pouchitis: A comparative proteomic approach

doi:10.3748/wjg.v16.i1.30

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Jan 7, 2010 (publication date) through Nov 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jan 7, 2010; 16(1): 30-41
Published online Jan 7, 2010. doi: 10.3748/wjg.v16.i1.30

Antibiotics and probiotics in chronic pouchitis: A comparative proteomic approach

Silvia Turroni, Beatrice Vitali, Marco Candela, Paolo Gionchetti, Fernando Rizzello, Massimo Campieri, Patrizia Brigidi

Silvia Turroni, Beatrice Vitali, Marco Candela, Patrizia Brigidi, Department of Pharmaceutical Sciences, University of Bologna, via Belmeloro 6, 40126 Bologna, Italy

Paolo Gionchetti, Fernando Rizzello, Massimo Campieri, Department of Internal Medicine and Gastroenterology, University of Bologna, Polyclinic S. Orsola, via Massarenti 9, 40138 Bologna, Italy

Author contributions: Gionchetti P, Rizzello F and Campieri M directed patient recruitment and follow up; Turroni S, Vitali B and Candela M performed the research; all authors contributed to editing of the manuscript and approved the final version of the paper.

Correspondence to: Patrizia Brigidi, Professor, Department of Pharmaceutical Sciences, University of Bologna, via Belmeloro 6, 40126 Bologna, Italy. patrizia.brigidi@unibo.it

Telephone: +39-51-2099743 Fax: +39-51-2099734

Received: August 6, 2009
Revised: September 8, 2009
Accepted: September 15, 2009
Published online: January 7, 2010

Abstract

AIM: To profile protein expression in mucosal biopsies from patients with chronic refractory pouchitis following antibiotic or probiotic treatment, using a comparative proteomic approach.

METHODS: Two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry were used to characterize the changes related to antibiotic therapy in the protein expression profiles of biopsy samples from patients with chronic refractory pouchitis. The same proteomic approach was applied to identify differentially expressed proteins in the non-inflamed pouch before and after probiotic administration.

RESULTS: In the first set of 2D gels, 26 different proteins with at least 2-fold changes in their expression levels between the pouchitis condition and antibiotic-induced remission were identified. In the second set of analysis, the comparison between mucosal biopsy proteomes in the normal and probiotic-treated pouch resulted in 17 significantly differently expressed proteins. Of these, 8 exhibited the same pattern of deregulation as in the pouchitis/pouch remission group.

CONCLUSION: For the first time, 2D protein maps of mucosal biopsies from patients with ileal pouch-anal anastomosis were provided, and differentially expressed proteins following antibiotic/probiotic treatment were identified.

Keywords: Chronic disease; Pouchitis; Antibiotics; Probiotics; Proteins; Gene expression