Kadkhodaee M, Golab F, Zahmatkesh M, Ghaznavi R, Hedayati M, Arab HA, Ostad SN, Soleimani M. Effects of different periods of renal ischemia on liver as a remote organ. World J Gastroenterol 2009; 15(9): 1113-1118 [PMID: 19266605 DOI: 10.3748/wjg.15.1113]
Corresponding Author of This Article
Dr. Mehri Kadkhodaee, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, 14155-6447 Tehran, Iran. kadkhodm@tums.ac.ir
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World J Gastroenterol. Mar 7, 2009; 15(9): 1113-1118 Published online Mar 7, 2009. doi: 10.3748/wjg.15.1113
Effects of different periods of renal ischemia on liver as a remote organ
Mehri Kadkhodaee, Fereshteh Golab, Maryam Zahmatkesh, Rana Ghaznavi, Mehdi Hedayati, Hossein Ali Arab, Seyed Naser Ostad, Manoocher Soleimani
Mehri Kadkhodaee, Fereshteh Golab, Maryam Zahmatkesh, Rana Ghaznavi, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, 14155-6447 Tehran, Iran
Mehdi Hedayati, Endocrine and Metabolism Research Center, Shahid Beheshti University of Medical Sciences, 193954763 Tehran, Iran
Hossein Ali Arab, Department of Pharmacology, School of Veterinary Medicine, Tehran University, 141556453 Tehran, Iran
Seyed Naser Ostad, Cell culture laboratory, Faculty of Pharmacy, Tehran University of Medical Sciences, 14155-6447 Tehran, Iran
Manoocher Soleimani, Department of Medicine, University of Cincinnati, MSB G259 OH, United States
Author contributions: Kadkhodaee M conception and design, and drafting of the article; Zahmatkesh M conception and design, analysis and interpretation of data; Golab F acquisition, analysis and interpretation of data; Ghaznavi R, Hedayati M, Arab HA, Ostad SN acquisition of data; Soleimani M conception and design, final approval of the version to be published.
Correspondence to: Dr. Mehri Kadkhodaee, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, 14155-6447 Tehran, Iran. kadkhodm@tums.ac.ir
Telephone: +98-21-88259862
Fax: +98-21-66419484
Received: October 21, 2008 Revised: January 24, 2009 Accepted: January 31, 2009 Published online: March 7, 2009
Abstract
AIM: To assess the hepatic changes after induction of different periods of renal ischemia.
METHODS: Rats were subjected to either sham operation or ischemia (30, 45 and 60 min) followed by 60 min reperfusion. Liver and renal functional indices were measured. Hepatic glutathione (GSH) and ferric reducing antioxidant power levels and the concentration of interleukin (IL)-10 and tumor necrosis factor (TNF-α) were evaluated. Portions of liver and kidney tissues were fixed for histological evaluation.
RESULTS: Forty-five minutes renal ischemia followed by 60 min reperfusion caused significant changes in liver structure and a significant reduction in renal function. These rats showed a significant decrease in liver GSH, as well as a significant increase in TNF-α and IL-10 concentrations. These results demonstrated that renal ischemia caused changes in liver histology, function, oxidative stress and inflammatory status, which led to a reduction in hepatic antioxidant capacity. With 30 min ischemia, the magnitude of these changes was less than those with 45 or 60 min ischemia.
CONCLUSION: A minimum of 45 min ischemia is needed to study the effects of renal injury on the liver as a remote organ.
