Pradeep HA, Khan S, Ravikumar K, Ahmed MF, Rao MS, Kiranmai M, Reddy DS, Ahamed SR, Ibrahim M. Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies. World J Gastroenterol 2009; 15(38): 4816-4822 [PMID: 19824117 DOI: 10.3748/wjg.15.4816]
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Dr. Mohammed Ibrahim, Professor, PhD, Department of Pharmacology and Biotechnology, Nizam Institute of Pharmacy, Deshmukhi, Pochampally (Mandal), Near Ramoji Film City, Nalgonda 508284, Andhra Pradesh, India. ibrahim_cce@rediffmail.com
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World J Gastroenterol. Oct 14, 2009; 15(38): 4816-4822 Published online Oct 14, 2009. doi: 10.3748/wjg.15.4816
Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies
Hulikere Ananth Pradeep, Saleemullah Khan, Karamkonda Ravikumar, Mohammed Fazil Ahmed, Meesala Srinivasa Rao, Mandava Kiranmai, Dachani Sudhershan Reddy, Shaik Rasheed Ahamed, Mohammed Ibrahim
Hulikere Ananth Pradeep, Karamkonda Ravikumar, Mohammed Fazil Ahmed, Meesala Srinivasa Rao, Mandava Kiranmai, Dachani Sudhershan Reddy, Shaik Rasheed Ahamed, Mohammed Ibrahim, Department of Pharmacology and Biotechnology, Nizam Institute of Pharmacy, Deshmukhi, Pochampally (Mandal), Near Ramoji Film City, Nalgonda 508284, Andhra Pradesh, India
Saleemullah Khan, JSS College of Pharmacy, Ooty, Tamilnadu, India and Manipal College of Pharmaceutical Sciences, Manipal 576104, Karnataka, India
Mohammed Ibrahim, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences and Allied Hospitals, Kanchanbagh, Hyderabad 500058, Andhra Pradesh, India
Author contributions: Pradeep HA, Khan S, Ravikumar K and Rao MS designed research and performed research; Ahmed MF, Kiranmai M, Reddy DS and Ahamed SR helped write, organize, and correct the paper; the statistical data were analyzed by Khan S and Ibrahim M; Ibrahim M supervised the writing and organization process.
Correspondence to: Dr. Mohammed Ibrahim, Professor, PhD, Department of Pharmacology and Biotechnology, Nizam Institute of Pharmacy, Deshmukhi, Pochampally (Mandal), Near Ramoji Film City, Nalgonda 508284, Andhra Pradesh, India. ibrahim_cce@rediffmail.com
Telephone: +91-8685-202135 Fax: +91-8685-202135
Received: June 13, 2009 Revised: September 7, 2009 Accepted: September 14, 2009 Published online: October 14, 2009
Abstract
AIM: To evaluate the hepatoprotective activity of a hydroalcoholic extract of the bark of Anogeissus latifolia; in vitro in primary rat hepatocyte monolayer culture and in vivo in the liver of Wistar rats intoxicated by carbon tetrachloride (CCl4).
METHODS: In the in vitro study, a primary hepatocyte monolayer culture was treated with CCl4 and extract of Anogeissus latifolia. Hepatoprotective activity was demonstrated in the CCl4 damaged primary monolayer culture. In the in vivo study, the hepatoprotective activity of a hydroalcoholic extract of Anogeissus latifolia was analyzed in liver injured CCl4-treated rats. Biochemical parameters including serum transaminases [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)] and alkaline phosphatase (ALP) in serum were analyzed. The biochemical findings were supplemented with histopathological examination of rat liver sections.
RESULTS: In vitro: primary hepatocyte monolayer cultures were treated with CCl4 and extract of Anogeissus latifolia. A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo: Hydroalcoholic extract of Anogeissus latifolia (300 mg/kg) was found to have protective activity in rats with CCl4-induced liver damage as judged from serum marker enzyme activity.
CONCLUSION: The above findings lead to the conclusion that the hydroalcoholic extract of Anogeissus latifolia is hepatoprotective. Hence, we suggest that the inclusion of this plant in the management of liver disorders is justified.
