Brief Articles
Copyright ©2009 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Jan 14, 2009; 15(2): 219-225
Published online Jan 14, 2009. doi: 10.3748/wjg.15.219
Acute effects of Helicobacter pylori extracts on gastric mucosal blood flow in the mouse
Johanna Henriksnäs, Christer Atuma, Mia Phillipson, Stellan Sandler, Lars Engstrand, Lena Holm
Johanna Henriksnäs, Christer Atuma, Mia Phillipson, Stellan Sandler, Lena Holm, Department of Medical Cell Biology, Uppsala University, SE-751 23 Uppsala, Sweden
Lars Engstrand, Department of Bacteriology, Swedish Institute for Infectious Disease Control, 171 82 Solna, Sweden
Author contributions: Henriksnäs J, Atuma C and Phillipson M performed the experiments, Henriksnäs J, Atuma C, Phillipson M, Sandler S, Engstrand L and Holm L designed the experiments, Henriksnäs J and Holm L wrote the paper.
Correspondence to: Johanna Henriksnäs, Department of Medical Cell Biology, Uppsala University, PO Box 571, SE-751 23 Uppsala, Sweden. johanna.henriksnas@mcb.uu.se
Telephone: +46-18-4714183
Fax: +46-18-4714938
Received: September 5, 2008
Revised: December 3, 2008
Accepted: December 10, 2008
Published online: January 14, 2009
Abstract

AIM: To investigate the mechanisms underlying the reduction in gastric blood flow induced by a luminal water extract of Helicobacter pylori (HPE).

METHODS: The stomachs of isoflurane-anesthetized mice were exteriorized, and the mucosal surface exposed. Blood flow was measured with the laser-Doppler technique, and systemic arterial blood pressure monitored. C57BL/6 mice were exposed to water extract produced from H pylori strain 88-23. To investigate the role of a nerve- or iNOS-mediated pathway, we used intraluminal lidocaine and iNOS-/- mice. Blood flow response to the endogenous nitric oxide synthase inhibitor asymmetric dimethyl arginine (ADMA) was also assessed.

RESULTS: In wild-type mice, HPE decreased mucosal blood flow by approximately 30%. This reduction was abolished in iNOS-deficient mice, and by pre-treatment with lidocaine. Luminally applied ADMA resulted in reduction in blood flow similar to that observed in wild-type mice exposed to HPE.

CONCLUSION: A H pylori water extract reduces gastric mucosal blood flow acutely through iNOS- and nerve-mediated pathways.

Keywords: Gastric mucosal blood flow; In vivo; Laser-Doppler flowmetry; Mice; Helicobacter pylori