Orignal Articles
Copyright ©2009 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. May 14, 2009; 15(18): 2228-2233
Published online May 14, 2009. doi: 10.3748/wjg.15.2228
FAT10 level in human gastric cancer and its relation with mutant p53 level, lymph node metastasis and TNM staging
Feng Ji, Xi Jin, Chun-Hua Jiao, Qin-Wei Xu, Zi-Wei Wang, Yue-Liang Chen
Feng Ji, Xi Jin, Chun-Hua Jiao, Qin-Wei Xu, Zi-Wei Wang, Yue-Liang Chen, Department of Digestive Diseases, First Affiliated Hospital of Zhejiang University Medical School, Hangzhou 310003, Zhejiang Province, China
Author contributions: Ji F and Jin X designed the research and wrote the paper; Jiao CH, Xu QW, Wang ZW and Chen YL performed the research and analyzed the data.
Correspondence to: Feng Ji, Department of Digestive Diseases, First Affiliated Hospital of Zhejiang University Medical School, No. 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China. jifeng1126@sina.com
Telephone: +86-571-87236532
Fax: +86-571-87236611
Received: February 25, 2009
Revised: April 1, 2009
Accepted: April 8, 2009
Published online: May 14, 2009
Abstract

AIM: To investigate the role of FAT10 and mutant p53 in the pathogenesis, severity and prognosis of gastric cancer.

METHODS: FAT10, mutant p53 mRNA and protein levels were measured by reverse transcription (RT)-PCR and immunohistochemistry in gastric cancer tissue (n = 62), tumor-adjacent tissue (n = 62) and normal gastric tissue (n = 62). Relation of FAT10 and mutant p53 expression with clinicopathological features and clinical outcomes of gastric cancer patients were analyzed.

RESULTS: The FAT10, mutant p53 mRNA and protein levels were significantly higher in gastric cancer than in its adjacent and normal tissue. The FAT10 and mutant p53 levels in gastric cancer tissue were significantly correlated with lymph node metastasis and tumor, nodes, metastasis (TNM) staging. Moreover, the high FAT10 level was associated with the overall survival rate of patients. Multivariate Cox-proportional hazards model analysis showed that mRNA and protein levels of FAT10 and mutant p53, lymph node metastasis, distant metastasis and TNM stage were the independent prognostic factors for gastric cancer.

CONCLUSION: FAT10 may be involved in gastric carcinogenesis, and is a potential marker for the prognosis of gastric cancer patients. FAT10 and mutant p53 may play a common role in the carcinogenesis of gastric cancer.

Keywords: Gastric cancer; FAT10; Mutant p53; Prognosis