Original Articles
Copyright ©2009 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Apr 14, 2009; 15(14): 1738-1743
Published online Apr 14, 2009. doi: 10.3748/wjg.15.1738
Expression and location of α-fetoprotein during rat colon development
Ying-Bin Ge, Luo Gu, Jun Du, Peng Sun, Dan Dong, Xiao-Yan Liu
Xiao-Yan Liu, Dan Dong, Peng Sun, Jun Du, Luo Gu, Ying-Bin Ge, Department of Physiology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Author contributions: Liu XY and Dong D performed the animal treatment, RT-PCR, immunofluorescent staining and wrote the paper; Sun P did part of RT-PCR and immunofluorescent staining; Du J conducted Western blot and statistical analysis; Ge YB designed the experiment and wrote the paper; Gu L and Ge YB contributed equally to this work.
Correspondence to: Ying-Bin Ge, MD, PhD, Department of Physiology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. ybge@njmu.edu.cn
Telephone: +86-25-86862016
Fax: +86-25-86862016
Received: December 11, 2008
Revised: February 17, 2009
Accepted: February 24, 2009
Published online: April 14, 2009
Abstract

AIM: To investigate the expression of α-fetoprotein (AFP), a cancer-associated fetal glycoprotein, and its involvement during rat colon development.

METHODS: Colons from Sprague-Dawley rat fetuses, young and adult (8 wk old) animals were used in this study. Expression levels of AFP in colons of different development stage were detected by reverse-transcriptase PCR (RT-PCR) and Western blotting. To identify the cell location of AFP in the developing rat colons, double-immunofluorescent staining was performed using antibodies to specific cell markers and AFP, respectively.

RESULTS: The highest levels of AFP mRNA were detected in colons of rats at embryonic day 18.5 (e18.5). Compared to e18.5 d, the AFP expression was significantly decreased during rat development [85% for e20.5, P < 0.05, 58% for postnatal day 0.5 (P0.5), P < 0.05, 37% for P7, P < 0.05, 24% for P14, P < 0.05, and 11% for P21, P < 0.05] and undetected in adult rats. Only the 72-kDa isoform of AFP was detected by Western blotting, the expression pattern was similar to AFP mRNA and conformed to the results of mRNA expression. The AFP positive staining was identical to different distribution patterns in fetuses, young and adult animals and positive staining for both AFP and vimentin was overlapped in mesenchymal cells at each stage tested.

CONCLUSION: This study has for the first time demonstrated that AFP is localized in the mesenchyme of rat colon from the embryo to the weaning stage by immunofluorescence and presents 72-kDa isoform in the developing rat colons by Western blotting. The dynamic expression of AFP in the various developmental stages of the colon indicates that AFP might be involved in many aspects of colon development.

Keywords: Alpha-fetoprotein; Development; Mesenchyme; Colon; Rat