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World J Gastroenterol. Feb 28, 2008; 14(8): 1263-1267
Published online Feb 28, 2008. doi: 10.3748/wjg.14.1263
Efficacies of β-L-D4A against hepatitis B virus in 2.2.15 cells
Lin-Lin Gao, Xiao-Yan Wang, Ju-Sheng Lin, Ying-Hui Zhang, Yan Li
Lin-Lin Gao, Xiao-Yan Wang, Ju-Sheng Lin, Ying-Hui Zhang, Yan Li, Institute of Liver Diseases, Tongji Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Author contributions: Gao LL and Wang XY contributed equally to this work; Gao LL, Wang XY, Lin JS, Zhang YH, Li Y designed the research; Gao LL and Wang XY performed the research; Gao LL wrote the paper
Correspondence to: Professor Ju-Sheng Lin, Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. linjusheng2001@163.com
Telephone: +86-27-83663661
Fax: +86-27-83646605
Received: September 17, 2007
Revised: December 18, 2007
Published online: February 28, 2008
Abstract

AIM: To investigate the antiviral effect of beta-L-enantiomer of 2’,3’-didehydro-2’,3’-dideoxyadenosine (β-L-D4A) on 2.2.15 cells transfected with the hepatitis B virus (HBV) genome.

METHODS: Lamivudine (3TC) as a positive control. Then, HBV DNA in treated 2.2.15 cells and the Hepatitis B surface antigen (HBsAg) in the culture supernatants were detected to determine the inhibitory effect of β-L-D4A. At the same time, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to detect the survival ratio of 2.2.15 cells.

RESULTS: β-L-D4A has a dose-dependent inhibitory effect on HBV DNA replication; this effect was apparent when the concentration was above 1 mol/L. When β-L-D4A was at the highest concentration, 100 mol/L, the HBsAg inhibition ratio was above 50%. The Therapeutic index (TI) of β-L-D4A was above 2.1.

CONCLUSION: β-L-D4A has a dose-dependent inhibitory effect on the replication of HBV DNA and the secretion of HBsAg at low toxicity.

Keywords: 2’,3’-didehydro-2’,3’-dideoxyadenosine; 2.2.15 cells; Hepatitis B virus DNA; Hepatitis B surface antigen