Cao SY, Wang MS, Cheng AC, Qi XF, Yang XY, Deng SX, Yin NC, Zhang ZH, Zhou DC, Zhu DK, Luo QH, Chen XY. Comparative analysis of intestinal microbial community diversity between healthy and orally infected ducklings with Salmonella enteritidis by ERIC-PCR. World J Gastroenterol 2008; 14(7): 1120-1125 [PMID: 18286697 DOI: 10.3748/wjg.14.1120]
Corresponding Author of This Article
Professor An-Chun Cheng, Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China. chenganchun@vip.163.com
Article-Type of This Article
Rapid Communication
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Sheng-Yan Cao, Xue-Feng Qi, Xiao-Yan Yang, Shu-Xuan Deng, Nian-Chun Yin, Zhen-Hua Zhang, Deng-Chun Zhou, Qi-Hui Luo, Xiao-Yue Chen, Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China
An-Chun Cheng, Ming-Shu Wang, De-Kang Zhu, Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Yaan 625014, Sichuan Province, China
Ming-Shu Wang, College of Life Science and Technology of Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China
Author contributions: Cao SY, Cheng AC, Wang MS contributed equally to this work and designed the research; Cao SY, Qi XF performed the research; Yang XY, Deng SX, Yin NC, Zhang ZH, Zhou DC, Luo QH and Chen XY provided new reagents/analytic tools; Cao SY analyzed data and wrote the paper.
Correspondence to: Professor An-Chun Cheng, Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China. chenganchun@vip.163.com
Telephone: +86-835-2885774
Fax: +86-835-2885774
Received: November 15, 2007 Revised: December 24, 2007 Published online: February 21, 2008
Abstract
AIM: To analyze the difference of intestinal microbial community diversity between healthy and (S. enteritidis) orally infected ducklings.
METHODS: Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was applied to analyze the intestinal microbial community diversity and dynamic change including duodenum, jejunum, ileum, cecum and rectum from healthy ducklings and 7-day-old ducklings after oral infection with S. enteritidis at different time points.
RESULTS: The intestinal microbial community of the control healthy ducklings was steady and the ERIC-PCR band numbers of the control healthy ducklings were the least with rectum and were the most with caecum. ERIC-PCR bands of orally inoculated ducklings did not obviously change until 24 h after inoculation (p.i.). The numbers of the ERIC-PCR bands gradually decreased from 24 h to 72 h p.i., and then, with the development of disease, the band numbers gradually increased until 6 d p.i. The prominent bacteria changed because of S. enteritidis infection and the DNAstar of staple of ERIC-PCR showed that aerobe and facultative aerobe (Escherichia coli, Shigella, Salmonella) became preponderant bacilli in the intestine of orally infected ducklings with SE.
CONCLUSION: This study has provided significant data to clarify the intestinal microbial community diversity and dynamic change of healthy and S. enteritidis orally infected ducklings, and valuable insight into the pathogenesis of S. enteritidis infection in both human and animals.
