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World J Gastroenterol. Feb 7, 2008; 14(5): 776-781
Published online Feb 7, 2008. doi: 10.3748/wjg.14.776
Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues
Bin Yan, An-Chun Cheng, Ming-Shu Wang, Shu-Xuan Deng, Zhen-Hua Zhang, Nian-Chun Yin, Ping Cao, Sheng-Yan Cao
Bin Yan, Shu-Xuan Deng, Zhen-Hua Zhang, Nian-Chun Yin, Ping Cao, Sheng-Yan Cao, Avian Disease Research Center, Collage of Veterinary Medicine of Sichuan Agriculture University, Yaan 625014, Sichuan Province, China
Ming-Shu Wang, College of Life Science and Technology of Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China
An-Chun Cheng, Ming-Shu Wang, Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine of Sichuan Agriculture University, Yaan 625014, Sichuan Province, China
Correspondence to: Professor An-Chun Cheng, Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Animal Science and Veterinary Medicine of Sichuan Agriculture University, Yaan 625014, Sichuan Province, China. chenganchun@vip.163.com
Telephone: +86-835-2885774
Fax: +86-835-2885774
Received: August 30, 2007
Revised: November 15, 2007
Published online: February 7, 2008
Abstract

AIM: To detect Salmonella enteritidis (S. enteritidis) in paraffin slices and antigen location in infected duck tissues.

METHODS: Rabbits were immunized with purified bacillus to obtain S. enteritidis-specific antibody, which were then extracted by the caprylic-ammonium sulphate method, purified through High-Q columns. An indirect immuno-fluorescent staining method (IFA) was established to detect the S. enteritidis antigen in paraffin slices. S. enteritidis was detected in each organ tissue of ducklings experimentally infected with S. enteritidis.

RESULTS: The gland of Garder, heart, kidney, spleen, liver, brain, ileum, jejunum, bursa of Fabricius from S. enteritidis experimentally infected ducklings were positive or strongly positive, and the S. enteritidis antigen was mainly distributed in the infected cell cytoplasm.

CONCLUSION: IFA is an intuitionist, sensitive and specific method in detecting S. enteritidis antigen in paraffin wax slices, and it is a good method in diagnosis and antigen location of S. enteritidis. We also conclude that the gland of Garder, heart, kidney, spleen, liver, ileum, jejunum are target organs in S. enteritidis infections of duck, and S. enteritidis is an intracellular parasitic bacterium.

Keywords: Indirect immuno-fluorescent antibody staining; Salmonella enteritidis; Paraffin tissues; Detection; Localization of antigens