Helicobacter Pylori
Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Dec 7, 2008; 14(45): 6924-6928
Published online Dec 7, 2008. doi: 10.3748/wjg.14.6924
Helicobacter pylori damages human gallbladder epithelial cells in vitro
Dong-Feng Chen, Lu Hu, Ping Yi, Wei-Wen Liu, Dian-Chun Fang, Hong Cao
Dong-Feng Chen, Lu Hu, Ping Yi, Wei-Wen Liu, Dian-Chun Fang, Hong Cao, Department of Gastroenterology, Daping Hospital, Third Military Medical University, Chongqing 400042, China
Supported by The National Natural Science Foundation of China, No. 39970039
Correspondence to: Dr. Dong-Feng Chen, Department of Gastroenterology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China. dfchen9@hotmail.com.cn
Telephone: +86-23-68757342 Fax: +86-23-68813806
Received: August 5, 2005
Revised: November 12, 2008
Accepted: November 19, 2008
Published online: December 7, 2008
Abstract

AIM: To study the mechanism by which Helicobacter pylori (H pylori) damages human gallbladder epithelial cells (HGBEC).

METHODS: H pylori isolated from gallbladder were cultured in a liquid medium. Different concentration supernatants and sonicated extracts of H pylori cells were then added to HGBEC in a primary culture. The morphological changes in HGBEC as well as changes in the levels of alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and glutamyltransferase (GGT) were measured.

RESULTS: According to the culture curve of HGBEC, it was convenient to study the changes in HGBEC by adding H pylori sonicated extracts and H pylori culture supernatants. Both H pylori sonicated extracts and H pylori culture supernatants had a significant influence on HGBEC morphology, i.e. HGBEC grew more slowly, their viability decreased and their detachment increased. Furthermore, HGBEC ruptured and died. The levels of ALP (33.84 ± 6.00 vs 27.01 ± 4.67, P < 0.05), LDH (168.37 ± 20.84 vs 55.51 ± 17.17, P < 0.01) and GGT (42.01 ± 6.18 vs 25.34 ± 4.33, P < 0.01) significantly increased in the HGBEC culture supernatant in a time- and concentration-dependent. The damage to HGBEC in H pylori culture liquid was more significant than that in H pylori sonicated extracts.

CONCLUSION: H pylori induces no obvious damage to HGBEC.

Keywords: Alkaline phosphatase; Glutamyltransferase; Helicobacter pylori; Human gallbladder epithelial cells; Lactate dehydrogenase