Basic Research
Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Nov 21, 2008; 14(43): 6681-6688
Published online Nov 21, 2008. doi: 10.3748/wjg.14.6681
Portal venous arterialization resulting in increased portal inflow and portal vein wall thickness in rats
Wen-Gang Li, Yong-Liang Chen, Jing-Xi Chen, Lei Qu, Bin-Dang Xue, Zhi-Hai Peng, Zhi-Qiang Huang
Wen-Gang Li, Jing-Xi Chen, Department of Hepatobiliary Pancreas and Vessel Surgery, Xiamen First Hospital Attached Fujian Medical University, Xiamen 361003, Fujian Province, China
Yong-Liang Chen, Zhi-Qiang Huang, Institute of Hepatobiliary Surgery, General Hospital of PLA, Beijing 100853, China
Lei Qu, Zhi-Hai Peng, Department of General Surgery, Shanghai First People’s Hospital, Shanghai 200080, China
Bin-Dang Xue, Image Processing Centre, Beijing University of Aeronautics and Astronautics, Beijing 100083, China
Author contributions: Li WG was responsible for study design, data collection and writing the paper; Chen YL was responsible study design and data collection; Chen JX and Qu L were responsible for data collection; Xue BD, Peng ZH and Huang ZQ was responsible for study design.
Supported by Science and Technology Plan of Xiamen City, No. 3502Z20064005; and Health Bureau of Xiamen City, No. WSk0521
Correspondence to: Yong-Liang Chen, Institute of Hepatobiliary Surgery, General Hospital of PLA, Beijing 100853, China. zf.23380681@163.com
Telephone: +86-24-23380681 Fax: +86-24-23380681
Received: August 2, 2008
Revised: September 27, 2008
Accepted: October 3, 2008
Published online: November 21, 2008
Abstract

AIM: To explore the influence of portal vein hemo-dynamic changes after portal venous arterialization (PVA) on peribiliary vascular plexus (PVP) morphological structure and hepatic pathology, and to establish a theoretical basis for the clinical application of PVA.

METHODS: Sprague-Dawley rats were randomly divided into control and PVA groups. After PVA, hemodynamic changes of the portal vein and morphological structure of hepatohilar PVP were observed using Doppler ultrasound, liver function tests, ink perfusion transparency management and three-dimensional reconstruction of computer microvisualization, and pathological examination was performed on tissue from the bile duct wall and the liver.

RESULTS: After PVA, the cross-sectional area and blood flow of the portal vein were increased, and the increase became more significant over time, in a certain range. If the measure to limit the flow in PVA was not adopted, the high blood flow would lead to dilatation of intrahepatic portal vein and its branches, increase in collagen and fiber degeneration in tunica intima. Except glutamic pyruvic transaminase (GPT), other liver function tests were normal.

CONCLUSION: Blood with a certain flow and oxygen content is important for filling the PVP and meeting the oxygen requirement of the bile duct wall. After PVA, It is the anatomic basis to maintain normal morphology of hepatohilar bile duct wall that the blood with high oxygen content and high flow in arterialized portal vein may fill PVP by collateral vessel reflux. A adequate measure to limit blood flow is necessary in PVA.

Keywords: Peribiliary vascular plexus; Portal venous arterialization; Liver transplantation; Bile duct neoplasms; Three-dimensional reconstruction; Hemodynamics