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World J Gastroenterol. Nov 14, 2008; 14(42): 6564-6568
Published online Nov 14, 2008. doi: 10.3748/wjg.14.6564
Low level of galacto-oligosaccharide in infant formula stimulates growth of intestinal Bifidobacteria and Lactobacilli
Xiao-Ming Ben, Juan Li, Zong-Tai Feng, Sheng-Yun Shi, Ya-Dong Lu, Rui Chen, Xiao-Yu Zhou
Xiao-Ming Ben, Zong-Tai Feng, Ya-Dong Lu, Rui Chen, Xiao-Yu Zhou, Department of Neonatology, Nanjing Children’s Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
Juan Li, Department of Pediatric Immunology, Nanjing Children’s Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
Sheng-Yun Shi, Pediatric Research Institute, Nanjing Children’s Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
Author contributions: Ben XM and Li J contributed equally to this work; Ben XM designed the research; Li J, Feng ZT, Lu YD, Chen R, Zhou XY performed the research; Shi SY contributed to the new reagents/analytic tools; Ben XM and Li J analyzed the data, and wrote the paper.
Correspondence to: Dr. Xiao-Ming Ben, Department of Neonatology, Nanjing Children’s Hospital of Nanjing Medical University, 72 Guangzhou Road, Nanjing 210008, Jiangsu Province, China. benxm@163.com
Telephone: +86-25-51554501 Fax: +86-25-83304239
Received: July 11, 2007
Revised: October 13, 2008
Accepted: October 20, 2008
Published online: November 14, 2008
Abstract

AIM: To investigate the effect of a new infant formula supplemented with a low level (0.24 g/100 mL) of galacto-oligosaccharide (GOS) on intestinal micro-flora (Bifidobacteria, Lactobacilli and E. coli) and fermentation characteristics in term infants, compared with human milk and a standard infant formula without GOS.

METHODS: Term infants (n = 371) were approached in this study in three hospitals of China. All infants started breast-feeding. Those who changed to formula-feeding within 4 wk after birth were randomly assigned to one of the two formula groups. Growth and stool characteristics, and side effects that occurred in recruited infants were recorded in a 3-mo follow-up period. Fecal samples were collected from a subpopulation of recruited infants for analysis of intestinal bacteria (culture technique), acetic acid (gas chromatography) and pH (indicator strip).

RESULTS: After 3 mo, the intestinal Bifidobacteria, Lactobacilli, acetic acid and stool frequency were significantly increased, and fecal pH was decreased in infants fed with the GOS-formula or human milk, compared with those fed with the formula without GOS. No significant differences were observed between the GOS formula and human milk groups. Supplementation with GOS did not influence the incidence of crying, regurgitation and vomiting.

CONCLUSION: A low level of GOS (0.24 g/100 mL) in infant formula can improve stool frequency, decrease fecal pH, and stimulate intestinal Bifidobacteria and Lactobacilli as in those fed with human milk.

Keywords: Human milk; Prebiotic; Probiotic; Safety; Chinese