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World J Gastroenterol. Nov 7, 2008; 14(41): 6318-6326
Published online Nov 7, 2008. doi: 10.3748/wjg.14.6318
Different effects of ghrelin, des-acyl ghrelin and obestatin on gastroduodenal motility in conscious rats
Mineko Fujimiya, Akihiro Asakawa, Koji Ataka, Ikuo Kato, Akio Inui
Mineko Fujimiya, Koji Ataka, Department of Anatomy, Sapporo Medical University, School of Medicine, Sapporo 060-8556, Japan
Akihiro Asakawa, Akio Inui, Department of Behavioral Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
Koji Ataka, Research Institute, Taiko Pharmaceutical Co., Ltd. Osaka, Japan
Ikuo Kato, Department of Bioorganic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan
Author contributions: Fujimiya M, Asakawa A and Inui A designed research; Ataka K performed research; Kato I synthesized peptides; Fujimiya M wrote the paper.
Correspondence to: Mineko Fujimiya, Department of Anatomy, Sapporo Medical University, School of Medicine, Chuo-ku, Sapporo 060-8556, Japan. fujimiya@sapmed.ac.jp
Telephone: +81-11-6112111-2640 Fax: +81-11-6184288
Received: October 15, 2008
Revised: October 23, 2008
Accepted: October 30, 2008
Published online: November 7, 2008
Abstract

Three peptides, ghrelin, des-acyl ghrelin and obestatin are derived from a common prohormone, preproghrelin by posttranslational processing, originating from endocrine cells in the stomach. To examine the effects of these peptides, we applied the manometric measurement of gastrointestinal motility in freely moving conscious rat models. Ghrelin exerts stimulatory effects on the motility of antrum and duodenum in both fed and fasted state of animals. Des-acyl ghrelin exerts inhibitory effects on the motility of antrum, but not on the motility of duodenum in the fasted state of animals. Obestatin exerts inhibitory effects on the motility of antrum and duodenum in the fed state, but not in the fasted state of animals. NPY Y2 or Y4 receptors in the brain may mediate the action of ghrelin, CRF type 2 receptors in the brain mediate the action of des-acyl ghrelin, whereas CRF type 1 and type 2 receptors in the brain mediate the action of obestatin. Vagal afferent pathways might be involved in the action of ghrelin, but not involved in the action of des-acyl ghrelin, whereas vagal afferent pathways might be partially involved in the action of obestatin.

Keywords: Ghrelin; Des-acyl ghrelin; Obestatin; Gastrointestinal motility; Hypothalamus