Chauhan R, Kazim SN, Kumar M, Bhattacharjee J, Krishnamoorthy N, Sarin SK. Identification and characterization of genotype A and D recombinant hepatitis B virus from Indian chronic HBV isolates. World J Gastroenterol 2008; 14(40): 6228-6236 [PMID: 18985816 DOI: 10.3748/wjg.14.6228]
Corresponding Author of This Article
Dr. Shiv Kumar Sarin, MD, Director Professor and Head, Department of Gastroenterology, G. B. Pant Hospital, Delhi University Affiliated, Room No. 201, Academic Block, New Delhi 110002, India. sksarin@nda.vsnl.net.in
Article-Type of This Article
Rapid Communication
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Ranjit Chauhan, Manoj Kumar, Shiv Kumar Sarin, Department of Gastroenterology and Advanced Center for Liver Diseases, G.B. Pant Hospital, New Delhi 110002, India
Syed Naqui Kazim, Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India
Ranjit Chauhan, Jayashree Bhattacharjee, Department of Biochemistry, Lady Hardinge Medical College, New Delhi 110002, India
Narayanasamy Krishnamoorthy, The Centre for Genomic Application, New Delhi 110002, India
Author contributions: Chauhan R and Sarin SK designed research; Chauhan R and Kumar M performed research; Krishnamoorthy N contributed reagents/analytic tools; Chauhan R and Kazim SN analyzed data; Chauhan R, Bhattacharjee J and Sarin SK wrote the paper.
Supported by Indian Council of Medical Research-Advanced Center for Liver Diseases Project (ICMR-ACLD)
Correspondence to: Dr. Shiv Kumar Sarin, MD, Director Professor and Head, Department of Gastroenterology, G. B. Pant Hospital, Delhi University Affiliated, Room No. 201, Academic Block, New Delhi 110002, India. sksarin@nda.vsnl.net.in
Telephone: +91-11-23232013 Fax: +91-11-23239710
Received: May 12, 2008 Revised: June 23, 2008 Accepted: June 30, 2008 Published online: October 28, 2008
Abstract
AIM: To confirm the presence of recombination, full-length hepatitis B virus (HBV) from chronic patients was sequenced and analyzed.
METHODS: Full-length HBV genomes from 12 patients were amplified and sequenced in an automated sequencer. Phylogenetic analysis was carried out on full-length, Core and preS2/Surface regions using MEGA software. SimPlot Boot Scanning and amino acid sequence analysis were performed for confirmation of recombination.
RESULTS: Eight patients were infected with genotype D strain; one patient with genotype A and three patients had genotype A and D recombination; two of them had cirrhosis and one had hepatocellular carcinoma. Phylogenetic analysis of core and preS2/surface regions separately showed evidence of genotype A and D recombination. The breakpoints of recombination were found to be at the start of preS2 and at the end of surface coding regions.
CONCLUSION: We identified and characterized recombinant A and D genotype HBV in hepatitis B surface antigen (HBsAg)-positive patients.
