Youn MJ, Kim JK, Park SY, Kim Y, Kim SJ, Lee JS, Chai KY, Kim HJ, Cui MX, So HS, Kim KY, Park R. Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells. World J Gastroenterol 2008; 14(4): 511-517 [PMID: 18203281 DOI: 10.3748/wjg.14.511]
Corresponding Author of This Article
Raekil Park, MD, PhD, Vestibulocochlear Research Center, Wonkwang University School of Medicine, #344-2, Shinyoung-dong, Iksan, Jeonbuk 570-749, Korea. rkpark@wku.ac.kr
Article-Type of This Article
Liver Cancer
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World J Gastroenterol. Jan 28, 2008; 14(4): 511-517 Published online Jan 28, 2008. doi: 10.3748/wjg.14.511
Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells
Myung-Ja Youn, Jin-Kyung Kim, Seong-Yeol Park, Yunha Kim, Se-Jin Kim, Jin Seok Lee, Kyu Yun Chai, Hye-Jung Kim, Ming-Xun Cui, Hong Seob So, Ki-Young Kim, Raekil Park
Myung-Ja Youn, Jin-Kyung Kim, Seong-Yeol Park, Yunha Kim, Se-Jin Kim, Hong Seob So, Raekil Park, Vestibulocochlear Research Center, Wonkwang University School of Medicine, Jeonbuk, Korea
Ki-Young Kim, Ming-Xun Cui, College of Human Environmental Science, Wonkwang University, Jeonbuk, Korea
Jin Seok Lee, Kyu Yun Chai, Department of Bionanochemistry, College of Natural Science, Wonkwang University, Jeonbuk, Korea
Hye-Jung Kim, Department of Family Medicine, Wonkwang University Hospital, Wonkwang University, Jeonbuk, Korea
Correspondence to: Raekil Park, MD, PhD, Vestibulocochlear Research Center, Wonkwang University School of Medicine, #344-2, Shinyoung-dong, Iksan, Jeonbuk 570-749, Korea. rkpark@wku.ac.kr
Telephone: +82-63-8506777
Fax: +82-63-8520220
Received: July 16, 2007 Revised: October 18, 2007 Published online: January 28, 2008
Abstract
AIM: To investigate the anti-proliferative and apoptotic effects of Chaga mushroom (Inonotus obliquus) water extract on human hepatoma cell lines, HepG2 and Hep3B cells.
METHODS: The cytotoxicity of Chaga extract was screened by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Morphological observation, flow cytometry analysis, Western blot were employed to elucidate the cytotoxic mechanism of Chaga extract.
RESULTS: HepG2 cells were more sensitive to Chaga extract than Hep3B cells, as demonstrated by markedly reduced cell viability. Chaga extract inhibited the cell growth in a dose-dependent manner, which was accompanied with G0/G1-phase arrest and apoptotic cell death. In addition, G0/G1 arrest in the cell cycle was closely associated with down-regulation of p53, pRb, p27, cyclins D1, D2, E, cyclin-dependent kinase (Cdk) 2, Cdk4, and Cdk6 expression.
CONCLUSION: Chaga mushroom may provide a new therapeutic option, as a potential anticancer agent, in the treatment of hepatoma.
