Basic Research
Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Oct 7, 2008; 14(37): 5683-5688
Published online Oct 7, 2008. doi: 10.3748/wjg.14.5683
Protective effect of ilomastat on trinitrobenzenesulfonic acid-induced ulcerative colitis in rats
Ying-De Wang, Wei Wang
Ying-De Wang, Department of Gastroenterology, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China
Wei Wang, Department of Gastroenterology, the Affiliated Zhongshan Hospital of Dalian University, Dalian 116023, Liaoning Province, China
Author contributions: Wang YD designed the research; Wang W performed the research; Wang YD and Wang W analyzed the data; Wang YD wrote the paper.
Correspondence to: Dr. Ying-De Wang, Department of Gastroenterology, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China. albertwyd@yahoo.com.cn
Telephone: +86-411-83635963-3162 Fax: +86-411-83632844
Received: April 9, 2008
Revised: August 8, 2008
Accepted: August 15, 2008
Published online: October 7, 2008
Abstract

AIM: To evaluate the protective effects of ilomastat, an exogenous matrix metalloproteinase (MMP) inhibitor, on trinitrobenzenesulfonic acid (TNB)-induced ulcerative colitis (UC) in rats.

METHODS: Male SD rats were randomly divided into model group, protective groups A and B, and normal control group. Rats in the model group received only intra-colonic TNB. Rats in the protective groups A and B received intra-peritoneal ilomastat of 10 mg/kg and 20 mg/kg, respectively, beside TNB. Rats in the normal control group received only intra-colonic normal saline. After 3 wk, segments of colon were obtained. RT-PCR and immunohistochemistry were used to examine the expression of MMP-1 and TIMP-1. Hematoxylin-eosin (HE) staining was used for pathological study.

RESULTS: The model of UC was successfully induced in rats. Inflammation of colonic mucosa greatly improved in protective groups A and B. Expression of MMP-1 and TIMP-1 in the model group, protective groups A and B was significantly higher than that in the normal control group (P < 0.0001) with MMP-1 expression increased more significantly than TIMP-1 expression. Expression of MMP-1 in protective groups A and B was significantly lower than that in the model group (P < 0.0001) . Expression of MMP-1 in protective group B was significantly lower than that in protective group A (P < 0.0001).

CONCLUSION: Ilomastat improves TNB-induced UC in rats by inhibiting the MMP-1 activity.

Keywords: Ulcerative colitis; Trinitrobenzenesulfonic acid; Matrix metalloproteinase-1; Tissue inhibitors of matrix metalloproteinase-1; Ilomastat