Yuan YH, Xiao EH, Liu JB, He Z, Jin K, Ma C, Xiang J, Xiao JH, Chen WJ. Gene expression and MR diffusion-weighted imaging after chemoembolization in rabbit liver VX-2 tumor model. World J Gastroenterol 2008; 14(36): 5557-5563 [PMID: 18810775 DOI: 10.3748/wjg.14.5557]
Corresponding Author of This Article
Dr. You-Hong Yuan, Department of Radiology, Hunan Province People’s Hospital, Changsha 410005, Hunan Province, China. heyuanyouhong@yahoo.com.cn
Article-Type of This Article
Liver Cancer
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World J Gastroenterol. Sep 28, 2008; 14(36): 5557-5563 Published online Sep 28, 2008. doi: 10.3748/wjg.14.5557
Gene expression and MR diffusion-weighted imaging after chemoembolization in rabbit liver VX-2 tumor model
You-Hong Yuan, En-Hua Xiao, Jian-Bin Liu, Zhong He, Ke Jin, Cong Ma, Jun Xiang, Jian-Hua Xiao, Wei-Jian Chen
You-Hong Yuan, Jian-Bin Liu, Department of Radiology, Hunan Province People’s Hospital, Changsha 410005, Hunan Province, China
En-Hua Xiao, Zhong He, Cong Ma, Jun Xiang, Department of Radiology, the Second Xiang-Ya Hospital, Central South University, Changsha 410005, Hunan Province, China
Ke Jin, Wei-Jian Chen, Department of Pathology, Hunan Province Children’s Hospital, Changsha 410005, Hunan Province, China
Jian-Hua Xiao, Department of Epidemiology, Center for Disease Control of Hunan Province, Changsha 410005, Hunan Province, China
Author contributions: Yuan YH, Xiao EH designed research; Yuan YH, Xiao EH, Liu JB, He Z, Jin K, Ma C, Xiang J, Xiao JH, Chen WJ performed research; Yuan YH analyzed data; and Yuan YH, Xiao EH wrote the paper.
Supported by The National Natural Science Foundation of China, No. 30070235 and 30470508; The Natural Science Foundation of Hunan Province, No. 08JJ5043; The Science and Technology Foundation of Hunan Province, No. 06FJ3120 and 2007SK3072; and The Medical Science and Technology Foundation of Hunan Province, No. B2006-159
Correspondence to: Dr. You-Hong Yuan, Department of Radiology, Hunan Province People’s Hospital, Changsha 410005, Hunan Province, China. heyuanyouhong@yahoo.com.cn
Telephone: +86-731-2278047 Fax: +86-731-2278047
Received: March 3, 2008 Revised: August 26, 2008 Accepted: September 2, 2008 Published online: September 28, 2008
Abstract
AIM: To investigate the dynamic characteristics and the correlation between PCNA, Bax, nm23, E-cadherin expression and apparent diffusion coefficient (ADC) on MR diffusion-weighted imaging (DWI) after chemoembolization in rabbit liver VX-2 tumor model.
METHODS: Forty New Zealand rabbit liver VX-2 tumor models were included in the study. DWI was carried out periodically after chemoembolization. All VX-2 tumor samples in each group were examined by histopathology and Strept Avidin-Biotin Complex (SABC) immunohistochemical staining.
RESULTS: The PCNA expression index in VX-2 tumors was higher than in the normal parenchyma around the tumor (P < 0.001). Nm23, Bax or E-caderin expression index in VX-2 tumors were lower than in the normal parenchyma around the tumor (all P < 0.001). PCNA and nm23 expression in the VX-2 tumor periphery first increased and then decreased (P < 0.001 and P = 0.03, respectively), while the expression of Bax and E-cadherin before and after chemoembolization was insignificant. When b-value was 100 s/mm2, there was a linear correlation between PCNA expression and ADC in the area of VX-2 tumor periphery (P < 0.001), and PCNA expression in VX-2 tumor periphery influenced the ADC.
CONCLUSION: The potential of VX-2 tumor infiltrating and metastasizing decreases, while its ability to proliferate increases for a short time after chemoembolization. To some degree, the ADC value indirectly reflects the proliferation of VX-2 tumor cells.
