Yang YQ, Wu L, Chen JX, Sun JZ, Li M, Li DM, Lu HY, Su ZH, Lin XQ, Li JC. Relationship between nm23H1 genetic instability and clinical pathological characteristics in Chinese digestive system cancer patients. World J Gastroenterol 2008; 14(36): 5549-5556 [PMID: 18810774 DOI: 10.3748/wjg.14.5549]
Corresponding Author of This Article
Ji-Cheng Li, MD, Professor, Institute of Cell Biology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China. lijichen@zju.edu.cn
Article-Type of This Article
Gastric Cancer
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Author contributions: Li JC designed the research; Yang YQ, Wu L, Chen JX, Sun JZ, Li M, Li DM, Lu HY, Su ZH and Lin XQ performed the experiments; Wu L, Chen JX and Sun JZ contributed to the new reagents/ analytic tools; Yang YQ and Li JC analyzed the data; Yang YQ, Li M, Li DM and Li JC wrote the paper.
Supported by National Fund for Fostering Talents of Basic Sciences, No. J0730856; Scientific Research Foundation of Public Health Bureau of Zhejiang Province, No.2007A179, No. 2007B209, Foundation for Analyzing and Testing of Zhejiang Province, No. 03149
Correspondence to: Ji-Cheng Li, MD, Professor, Institute of Cell Biology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China. lijichen@zju.edu.cn
Telephone: +86-571-88208088 Fax: +86-571-88208094
Received: May 12, 2008 Revised: June 23, 2008 Accepted: June 30, 2008 Published online: September 28, 2008
Abstract
AIM: To study the relationship between nm23H1 gene genetic instability and its clinical pathological characteristics in Chinese digestive system cancer patients.
METHODS: Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to analyze the microsatellite instability (MSI) and loss of heterozygosity (LOH). Immunohistochemistry was employed to detect the expression of nm23H1.
RESULTS: The MSI was higher in TNM stageI + II than in stage III + IV of gastric, colonic and gallbladder carcinomas. The LOH was higher in TNM stage III + IV than in stageI + II of gastric, colonic and hepatocellular carcinomas. Lymphatic metastasis was also observed. The expression of nm23H1 protein was lower in TNM stage III + IV than in stageI + II of these tumors and in patients with lymphatic metastasis.The nm23H1 protein expression was higher in the LOH negative group than in the LOH positive group.
CONCLUSION: MSI and LOH may independently control the biological behaviors of digestive system cancers. MSI could serve as an early biological marker of digestive system cancers. Enhanced expression of nm23H1 protein could efficiently inhibit cancer metastasis and improve its prognosis. LOH mostly appears in late digestive system cancer.
