Garza-González E, Bocanegra-García V, Bosques-Padilla FJ, Flores-Gutiérrez JP, Moreno F, Perez-Perez GI. mRNA levels of TLR4 and TLR5 are independent of H pylori. World J Gastroenterol 2008; 14(34): 5306-5310 [PMID: 18785283 DOI: 10.3748/wjg.14.5306]
Corresponding Author of This Article
Elvira Garza-González, Departmento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Madero y Dr. Aguirre s/n, Colonia Mitras Centro, Monterrey 64460, Mexico. elvira_garza_gzz@yahoo.com
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Rapid Communication
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World J Gastroenterol. Sep 14, 2008; 14(34): 5306-5310 Published online Sep 14, 2008. doi: 10.3748/wjg.14.5306
mRNA levels of TLR4 and TLR5 are independent of H pylori
Elvira Garza-González, Virgilio Bocanegra-García, Francisco Javier Bosques-Padilla, Juan Pablo Flores-Gutiérrez, Francisco Moreno, Guillermo Ignacio Perez-Perez
Elvira Garza-González, Francisco Moreno, Departmento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico
Virgilio Bocanegra-García, Departmento de Biología Molecular y Bioingeniería, UAM Reynosa Aztlán, Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas 88740, Mexico
Francisco Javier Bosques-Padilla, Juan Pablo Flores-Gutiérrez, Hospital Universitario “Dr. José Eleuterio González”, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico
Guillermo Ignacio Perez-Perez, Department of Medicine and Microbiology, New York University School of Medicine, New York NY 10010, United States
Author contributions: Garza-González E designed and coordinated the study, optimized PCR conditions, and drafted the manuscript; Bocanegra-García V and Moreno F extracted RNAs and performed molecular assays; Flores-Gutiérrez JP performed histological examination and interpretation of results; Bosques-Padilla FJ recruited patients and carried out endoscopies; Pérez-Perez GI participated in the design, coordination of the study and revised the drafted manuscript.
Correspondence to: Elvira Garza-González, Departmento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Madero y Dr. Aguirre s/n, Colonia Mitras Centro, Monterrey 64460, Mexico. elvira_garza_gzz@yahoo.com
Telephone: +52-81-83294166 Fax: +52-81-83294166
Received: May 4, 2008 Revised: August 18, 2008 Accepted: August 25, 2008 Published online: September 14, 2008
Abstract
AIM: To determine if the presence H pylori or its virulence affect toll-like receptor 4 (TLR4) and TLR5 mRNA expression levels.
METHODS: For the in vivo assays, gastric biopsies were obtained from 40 patients and H pylori status was determined. For the in vitro assays, human gastric adenocarcinoma mucosal cells (AGS) were cultured in the presence or absence of twelve selected H pylori strains. H pylori strains isolated from culture-positive patients and selected strains were genotyped for cagA and vacA. The cDNA was obtained from mRNA extracted from biopsies and from infected AGS cells. TLR4 and TLR5 mRNA levels were examined by real-time PCR.
RESULTS: The presence of H pylori did not affect the mRNA levels of TLR4 or TLR5 in gastric biopsies. The mRNA levels of both receptors were not influenced by the vacA status (P > 0.05 for both receptors) and there were no differences in TLR4 or TLR5 mRNA levels among the different clinical presentations/histological findings (P > 0.05). In the in vitro assay, the mRNA levels of TLR4 or TLR5 in AGS cells were not influenced by the vacAs1 status or the clinical condition associated with the strains (P > 0.05 for both TLR4 and TLR5).
CONCLUSION: The results of this study show that the mRNA levels of TLR4 and TLR5 in gastric cells, both in vivo and in vitro, are independent of H pylori colonization and suggest that vacA may not be a significant player in the first step of innate immune recognition mediated by TLR4 or TLR5.
