Wu B, Zhu JS, Zhang Y, Shen WM, Zhang Q. Predictive value of MTT assay as an in vitro chemosensitivity testing for gastric cancer: One institution’s experience. World J Gastroenterol 2008; 14(19): 3064-3068 [PMID: 18494060 DOI: 10.3748/wjg.14.3064]
Corresponding Author of This Article
Dr. Qiang Zhang, MD, Department of Gastroenterology, Shanghai Sixth Hospital Affiliated to Shanghai Jiaotong University, Yishang Road 600, Shanghai 200223, China. qzsjtu@yahoo.cn
Article-Type of This Article
Rapid Communication
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World J Gastroenterol. May 21, 2008; 14(19): 3064-3068 Published online May 21, 2008. doi: 10.3748/wjg.14.3064
Predictive value of MTT assay as an in vitro chemosensitivity testing for gastric cancer: One institution’s experience
Bin Wu, Jin-Shui Zhu, Yi Zhang, Wei-Ming Shen, Qiang Zhang
Bin Wu, Yi Zhang, Wei-Ming Shen, Department of Pharmacy, Shanghai Sixth Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200223, China
Jin-Shui Zhu, Qiang Zhang, Department of Gastroenterology, Shanghai Sixth Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200223, China
Author contributions: Wu B and Zhang Q designed and performed the research; Zhu JS contributed to the statistical advices; Zhang Y supplied the MTT assay data; Shen WM offered the MTT assay advices.
Correspondence to: Dr. Qiang Zhang, MD, Department of Gastroenterology, Shanghai Sixth Hospital Affiliated to Shanghai Jiaotong University, Yishang Road 600, Shanghai 200223, China. qzsjtu@yahoo.cn
Telephone: +86-21-64369181
Fax: +86-21-64701361
Received: February 4, 2008 Revised: March 24, 2008 Published online: May 21, 2008
Abstract
AIM: To investigate the predictive clinical value of in vitro 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay for directing chemosensitivity in patients with gastric cancer.
METHODS: Results of a total of 353 consecutive patients with gastric cancer treated with MTT-directed chemotherapy or physician’s empirical chemotherapy from July 1997 to April 2003 were reviewed and analyzed retrospectively.
RESULTS: The overall 5-year survival rate of MTT-sensitive group (MSG) and control group (CG) was 47.5% and 45.1%, respectively. The results of subgroup analysis with Cox proportional-hazards model were favorable for the MSG-sensitive group. However, no statistically significant difference in survival rate was observed between the two groups.
CONCLUSION: Individualized chemotherapy based on in vitro MTT assay is beneficial, but needs to be confirmed by further randomized controlled trials.
