Yang JL, Wang MS, Cheng AC, Pan KC, Li CF, Deng SX. A simple and rapid method for extracting bacterial DNA from intestinal microflora for ERIC-PCR detection. World J Gastroenterol 2008; 14(18): 2872-2876 [PMID: 18473413 DOI: 10.3748/wjg.14.2872]
Corresponding Author of This Article
Professor An-Chun Cheng, Avian Diseases Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China. chenganchun@vip.163.com
Article-Type of This Article
Rapid Communication
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Jin-Long Yang, Chuan-Feng Li, Shu-Xuan Deng, Avian Diseases Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China
An-Chun Cheng, Ming-Shu Wang, Kang-Cheng Pan, Avian Diseases Research Center, College of Veterinary Medicine of Sichuan Agricultural University; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Yaan 625014, Sichuan Province, China
Ming-Shu Wang, College of Life Science and Technology of Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China
Author contributions: Yang JL, Wang MS, Cheng AC and Pan KC contributed equally to this work; Cheng AC, Wang MS designed the research; Yang JL, Pan KC, Li CF and Deng SX performed the research; Li CF and Deng SX analyzed the data; Yang JL, Cheng AC and Wang MS wrote the paper.
Correspondence to: Professor An-Chun Cheng, Avian Diseases Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China. chenganchun@vip.163.com
Telephone: +86-835-2885774
Fax: +86-835-2885774
Received: December 3, 2007 Revised: February 2, 2008 Published online: May 14, 2008
Abstract
AIM: To develop a simple and convenient method for extracting genomic DNA from intestinal microflora for enterobacterial repetitive intergenic consensus (ERIC)-PCR detection.
METHODS: Five methods of extracting bacterial DNA, including Tris-EDTA buffer, chelex-100, ultrapure water, 2% sodium dodecyl sulfate and 10% Triton-100 with and without sonication, were compared with the commercial fecal DNA extraction kit method, which is considered as the gold standard for DNA extraction. The comparison was based on the yield and purity of DNA and the indexes of the structure and property of micro-organisms that were reflected by ERIC-PCR.
RESULTS: The yield and purity of DNA obtained by the chelex method was similar to that obtained with the fecal DNA kit. The ERIC-PCR results obtained for the DNA extracted by the chelex method and those obtained for DNA extracted with the fecal DNA kit were basically the same.
CONCLUSION: The chelex method is recommended for ERIC-PCR experiments in view of its simplicity and cost-effectiveness; and it is suitable for extracting total DNA from intestinal micro-organisms, particularly for handling a large number of samples.
