Uçan HB, Kaplan M, Salman B, Yilmaz U, Mentes BB, Aybay C. Effect of oophorectomy and exogenous estrogen replacement on liver injury in experimental obstructive jaundice. World J Gastroenterol 2008; 14(18): 2818-2824 [PMID: 18473404 DOI: 10.3748/wjg.14.2818]
Corresponding Author of This Article
Bülent Salman, MD, Department of General Surgery, Gazi University School of Medicine, Besevler 06500, Ankara, Turkey. dr.bsalman@yahoo.com
Article-Type of This Article
Clinical Research
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World J Gastroenterol. May 14, 2008; 14(18): 2818-2824 Published online May 14, 2008. doi: 10.3748/wjg.14.2818
Effect of oophorectomy and exogenous estrogen replacement on liver injury in experimental obstructive jaundice
Hamdi Bülent Uçan, Mehmet Kaplan, Bülent Salman, Utku Yilmaz, B Bülent Mentes, Cemalettin Aybay
Hamdi Bülent Uçan, Department of General Surgery, Karaelmas University Medical School, Zonguldak 67100, Turkey
Mehmet Kaplan, Department of General Surgery, Gaziantep State Hospital, Gaziantep 27100, Turkey
Bülent Salman, Utku Yilmaz, B Bülent Mentes, Department of General Surgery, Gazi University Medical School, Ankara 06500, Turkey
Cemalettin Aybay, Department of Immunology, Gazi University Medical School, Ankara 06500, Turkey
Author contributions: Uçan HB, Kaplan M and Salman B designed research; Uçan HB, Kaplan M, Salman B and Yilmaz U performed research; Mentes BB and Aybay C contributed new analytic tools; Uçan HB, Salman B and Yilmaz U Mentes BB analyzed datas; Uçan HB, Salman B and Yilmaz U wrote the paper; Mentes BB and Aybay C supervised and revised the article.
Correspondence to: Bülent Salman, MD, Department of General Surgery, Gazi University School of Medicine, Besevler 06500, Ankara, Turkey. dr.bsalman@yahoo.com
Telephone: +90-312-2024196
Fax: +90-312-2124647
Received: December 5, 2007 Revised: March 26, 2008 Published online: May 14, 2008
Abstract
AIM: To investigate the role of estrogen on liver injury in an experimental obstructive jaundice model.
METHODS: Three groups of female rats were constituted; group 1 was oophorectomized and given E2 (n = 14), group 2 was oophorectomized and given placebo (n = 14), and group 3 was sham operated (n = 14). Fourteen days following constitution of bile duct ligation, all groups were compared in terms of serum tests, histopathologic parameters, and tissue levels of IFN-γ and IL-6.
RESULTS: The parameters representing both the injury and/or the reactive response and healing were more pronounced in groups 1 and 2 (χ2 = 17.2, χ2 = 10.20; χ2 = 12.4, P < 0.05). In the sham operated or E2 administered groups significantly lower tissue levels of IFN-γ and higher IL-6 levels were found. In contrast, high IFN-γ and low IL-6 tissue levels were found in the oophorectomized and placebo group (P < 0.001). Kupffer cell alterations were observed to be more pronounced in the groups 1 and 3 (χ2 = 6.13, P < 0.05).
CONCLUSION: Our study indicates that E2 impaired liver functions, accelerated both the liver damage and healing. In the conditions of bile duct obstruction, estrogen significantly changed the cytokine milieu in the liver.
