Colorectal Cancer
Copyright ©2007 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 21, 2007; 13(47): 6365-6369
Published online Dec 21, 2007. doi: 10.3748/wjg.v13.i47.6365
Analysis of the expression of coxsackievirus and adenovirus receptor in five colon cancer cell lines
Yassan Abdolazimi, Majid Mojarrad, Mehrdad Pedram, Mohammad Hossein Modarressi
Yassan Abdolazimi, Majid Mojarrad, Mehrdad Pedram, Mohammad Hossein Modarressi, Department of Medical Genetics, Medical Sciences/University of Tehran, Tehran, Iran
Mohammad Hossein Modarressi, Reproductive Biotechnology Research Center, Avesina Research Institute, Tehran, Iran
Author contributions: All authors contributed equally to the work.
Supported by the Funds from Medical Sciences/University of Tehran and Iranian Ministry of Health and Medical Education
Correspondence to: Dr. Mohammad Hossein Modarressi, Department of Medical Genetics, Medical Sciences/University of Tehran, PO Box 14155-6447, Tehran, Iran. modaresi@tums.ac.ir
Telephone: +98-21-88953005 Fax: +98-21-66404577
Received: July 17, 2007
Revised: September 11, 2007
Accepted: November 29, 2007
Published online: December 21, 2007
Abstract

AIM: To investigate the expression of coxsackievirus and adenovirus receptor (CAR) and adenovirus-mediated reporter gene transfer in five human colon cancer cell lines.

METHODS: Expression of CAR-specific mRNA and protein was analyzed by reverse transcriptase polymerase chain reaction and Western blotting, respectively. Adenovirus-based gene delivery was evaluated by infection of cells with adenoviral vector carrying the green fluorescent protein (GFP) gene.

RESULTS: All the colon cancer cell lines examined (HT29, LS180, SW480, SW948 and SW1116) expressed CAR full-length mRNA and an alternatively-spliced variant that lacks the transmembrane coding exon. All cell lines were detected as CAR-positive by Western blot analysis. Further, all cells we examined were efficiently infected with adenoviral vector-GFP.

CONCLUSION: The data indicated that the five colon cancer cell lines tested expressed adenovirus primary receptor and could be efficiently infected by adenoviral vectors. Therefore, these cell lines will be useful for adenovirus-based gene transfer and research.

Keywords: Coxsackievirus and adenovirus receptor; Adenoviral infection; Gene therapy