Gao XQ, Han JX, Xu ZF, Zhang WD, Zhang HN, Huang HY. Identification of the differential expressive tumor associated genes in rectal cancers by cDNA microarray. World J Gastroenterol 2007; 13(3): 341-348 [PMID: 17230600 DOI: 10.3748/wjg.v13.i3.341]
Corresponding Author of This Article
Jin-Xiang Han, Shandong Medical and Biotechnology Center, Shandong Academy of Medical Sciences; Key Laboratory for Biotech Drugs, Ministry of Health; Key Laboratory of Modern Drugs and Technology of Shandong Province; 89 Jingshi Road, Jinan 250062, Shandong Province, China. samshjx@sina.com
Article-Type of This Article
Colorectal Cancer
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Xue-Qin Gao, Jin-Xiang Han, Hua-Ning Zhang, Hai-Yan Huang, Key Laboratory for Biotech-Drug, Ministry of Health, Key Laboratory of Modern Drugs and Technology of Shandong Province, Shandong Medical and Biotechnology Center, Shandong Academy of Medical Sciences, Jinan 250062, Shandong Province, China
Zhong-Fa Xu, Department of Surgery, Shandong Tumor Hospital, Shandong Academy of Medical Sciences, Jinan 250117, Shandong Province, China
Wei-Dong Zhang, Department of Experimental Pathology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shandong Province, China
Author contributions: All authors contributed equally to the work.
Supported by Major Project of Shandong Provincial Science and Technology, No.011100105, and Shandong Natural Sciences Funding Committee, No. Y2003C03
Correspondence to: Jin-Xiang Han, Shandong Medical and Biotechnology Center, Shandong Academy of Medical Sciences; Key Laboratory for Biotech Drugs, Ministry of Health; Key Laboratory of Modern Drugs and Technology of Shandong Province; 89 Jingshi Road, Jinan 250062, Shandong Province, China. samshjx@sina.com
Telephone: +86-531-82919888 Fax: +86-531-82951586
Received: July 27, 2006 Revised: August 25, 2006 Accepted: September 26, 2006 Published online: January 21, 2007
Abstract
AIM: To identify tumor associated genes of rectal cancer and to probe the application possibility of gene expression profiles for the classification of tumors.
METHODS: Rectal cancer tissues and their paired normal mucosa were obtained from patients undergoing surgical resection of rectal cancer. Total RNA was extracted using Trizol reagents. First strand cDNA synthesis was indirectly labeled with aminoallyl-dUTP and coupled with Cy3 or Cy5 dye NHS mono-functional ester. After normalization to total spots, the genes which background subtracted intensity did not exceed 2 SD above the mean blank were excluded. The data were then sorted to obtain genes differentially expressed by ≥ 2 fold up or down in at least 5 of the 21 patients.
RESULTS: In the 21 rectal cancer patients, 23 genes were up-regulated in at least 5 samples and 15 genes were down-regulated in at least 5 patients. Hierachical cluster analysis classified the patients into two groups according to the clinicopathological stage, with one group being all above stage II and one group all below stage II.
CONCLUSION: The up-regulated genes and down-regulated genes may be molecular markers of rectal cancer. The expression profiles can be used for classification of rectal cancer.
