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Copyright ©2007 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 7, 2007; 13(25): 3517-3522
Published online Jul 7, 2007. doi: 10.3748/wjg.v13.i25.3517
RhoA and RhoC -siRNA inhibit the proliferation and invasiveness activity of human gastric carcinoma by Rho/PI3K/Akt pathway
Hua-Wen Sun, Shi-Lun Tong, Jie He, Qi Wang, Li Zou, Shu-Jing Ma, Hai-Yan Tan, Jian-Fei Luo, Hong-Xue Wu
Hua-Wen Sun, Shi-Lun Tong, Jie He, Li Zou, Shu-Jing Ma, Hai-Yan Tan, Jian-Fei Luo, Hong-Xue Wu, Department of Gastrointestinal Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Hubei Province Nature Science Foundation, No. 2005ABA121
Correspondence to: Hua-Wen Sun, MD, Department of Gastro-intestinal Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China. sunhuawen888@163.com
Telephone: +86-27-88041911-2211 Fax: +86-27-88042292
Received: April 19, 2007
Revised: April 20, 2007
Accepted: May 12, 2007
Published online: July 7, 2007
Abstract

AIM: To evaluate the effects of adenovirus-mediated gene transfer of RhoA siRNA and RhoC siRNA on proliferation and invasion of SGC7901 cells by Rho/ PI3K/Akt pathway.

METHODS: Plasmid of RhoA siRNA and RhoC siRNA were constructed and transfected into SGC7901 cells. siRNA and LY294002 (PI3K inhibitor) were designed as the control group. The mRNA and protein expressions of RhoA and RhoC were respectively detected with RT-PCR and western blotting. In order to find out the changes of proliferation and invasion power of SGC7901 cell lines, we analyzed the data by MTT, Boyden chamber and evaluated apoptosis of cell with flow cytometry. We treated BALB /C nude mice with RhoA and RhoC-siRNA, and tumor control rate (%) in nude mice was calculated.

RESULTS: RhoA and RhoC siRNA transfections specifically down-regulated the corresponding mRNA and protein levels in SGC7901 Cells.The experiment of permeated artificial basal membrane showed that the invasion power of SGC7901 cell lines are on the decline after treatment of Ad-RhoA and RhoC-siRNA (12.64 ±3.27 vs 87.38 ± 17.38, P < 0.05). The values of 490 nm wavelength light absorption were different in the five groups. The number of alive cells in the group of RhoA and RhoC-siRNA was lower than others in the 6th d (0.71 ± 0.01 vs 3.82 ± 0,11 P < 0.05). The apoptosis rate of transfected RhoA and RhoC-siRNA group with FACS were 19.07% ± 1.78 and there were significant differences between treated and control groups (19.07 ± 1.78% vs 1.23 ± 0.11%, P < 0.01). The tumor transplantation experiment in BALB/C nude mice showed intratumoral injection of RhoA or RhoC siRNA can inhibit tumor growth.

CONCLUSION: RhoA and RhoC siRNA gene therapy mediated by adenovirus may be useful for inhibiting growth and invasion of SGC7901 through a PI3K/Akt pathway. These results provide a novel therapeutic target in preventing gastric cancer cell invasion and metastasis.

Keywords: siRNA; Inhibition; Carcinoma; Proliferation; Invasiveness