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World J Gastroenterol. Jun 28, 2007; 13(24): 3364-3368
Published online Jun 28, 2007. doi: 10.3748/wjg.v13.i24.3364
The receptor for β2GP I on membrane of hepatocellular carcinoma cell line SMMC-7721 is annexin II
Pu-Jun Gao, Yang Shi, Yan-Hang Gao, Ya-Wen Liu, Yan Tan
Pu-Jun Gao, Yang Shi, Yan-Hang Gao, Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China
Ya-Wen Liu, Department of Epidemiology, School of Public Health, Jilin University, Changchun 130021, Jilin Province, China
Yan Tan, Department of Center Laboratory, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China
Author contributions: All authors contributed equally to the work.
Supported by The National Nature Science Foundation of China, No. 30070338
Correspondence to: Dr. Yan Tan, Department of Center Laboratory, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China. bfcty@public.cc.jl.cn
Telephone: +86-431-85613235
Received: March 19, 2007
Revised: April 3, 2007
Accepted: April 11, 2007
Published online: June 28, 2007
Abstract

AIM: To evaluate the receptor protein which can specifically bind to β2GP I on the membrane of hepatocellular carcinoma (HCC) cell line SMMC-7721, and to study the biological function of the receptor.

METHODS: Through β2GP I -affinity chromatography column, the peptid-polysome-mRNA complex, which can specially bind to β2GP I , stayed with the column and was separated from the whole polysome of liver cells, and then eluted and collected. Using cDNA synthesis kit and cDNA PCR kit, the corresponding cDNA was obtained and sequenced. RT-PCR was used to amplify annexin II , and flow cytometry was used to study the competitive binding of annexin II with β2GP I to SMMC-7721.

RESULTS: A total of 1.1 kb of the cDNA fragment of the specific binding protein of β2GP I on liver cell membrane was obtained. The sequence of cDNA shared high homology with human annexin II (98%). Annexin II was expressed on the membrane of SMMC-7721, and could compete with β2GP I for combining with SMMC-7721.

CONCLUSION: The receptor for β2GP I on membrane of SMMC-7721 cells is annexin II , which might bridge HBV to infect hepatocytes.

Keywords: β2-GlycoproteinI; Hepatocellular carcinoma cell, Human annexin II