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World J Gastroenterol. Jun 14, 2007; 13(22): 3117-3121
Published online Jun 14, 2007. doi: 10.3748/wjg.v13.i22.3117
Specific serum immunoglobulin G to H pylori and CagA in healthy children and adults (south-east of Iran)
A Jafarzadeh, MT Rezayati, M Nemati
A Jafarzadeh, MT Rezayati, M Nemati, Department of Immunology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Author contributions: All authors contributed equally to the work.
Correspondence to: Abdollah Jafarzadeh, Associate Professor of Immunology, Department of Immunology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. jafarzadeh14@yahoo.com
Telephone: +98-391-5234003 Fax: +98-391-5225209
Received: February 14, 2007
Revised: March 3, 2007
Accepted: March 15, 2007
Published online: June 14, 2007
Abstract

AIM: To evaluate the serologic IgG response to H pylori and CagA across age groups and in healthy children and adults.

METHODS: Totally, 386 children aged 1-15 years and 200 adults aged 20-60 years, were enrolled to study. The serum samples of participant were tested for presence of anti-H pylori and anti-CagA IgG by using ELISA method.

RESULTS: The seroprevalence of H pylori in adults was significantly higher than that observed in children (67.5% vs 46.6%; P < 0.000003). In children, the seropositivity rate in males (51.9%) was significantly (P < 0.05) higher than that observed in females (41.7%). The prevalence of serum anti-CagA antibody was 72.8% and 67.4% in infected children and adults, respectively. The mean titer of serum anti-CagA antibodies was significantly higher among children in comparison to adults (64.1 Uarb/mL vs 30.7; P < 0.03). In infected children and adults the prevalence of serum anti-CagA antibody was higher in males compared to females (78.4% vs 66.3%; P = 0.07 and 75.6% vs 54.71%; P < 0.04, respectively). The age-specific prevalence of anti-H pylori and anti-CagA antibody (in infected subjects) was 37.6% and 59.57% at age 1-5 years, 46.9% and 75% at age 6-10 years, 54.9% and 79.45% at age 11-15, 59.01% and 83.33% at age 20-30 years, 66.6% and 60.52% at age 31-40 years, 73.46% and 63.88% at age 41-50 years and 75.75% and 60% at age 51-60 years with mean titer of anti-CagA antibody of 75.94, 63.32, 57.11, 52.06, 23.62, 21.52 and 21.80 Uarb/mL, respectively. There was significant difference between mean serum anti-CagA antibody in age subgroups (P < 0.001).

CONCLUSION: These results showed that anti-H pylori and anti-CagA antibodies were common in the children and adults. The H pylori-specific antibodies influenced by age and sex of subjects. Moreover, it seems that males are more susceptible to infection with CagA+ strains compared to females. The seroprevalence of anti-CagA antibody was increased with age, up to 30 years and then decreased. It was also found that the magnitude of the IgG response to CagA decreased with advanced age.

Keywords: Seroprevalence; H pylori; Adults; Children; CagA; Iran