Immunotherapy of hepatoma with a monoclonal antibody against murine endoglin

doi:10.3748/wjg.v13.i17.2479

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May 7, 2007 (publication date) through May 5, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Liver Cancer

World J Gastroenterol. May 7, 2007; 13(17): 2479-2483
Published online May 7, 2007. doi: 10.3748/wjg.v13.i17.2479

Immunotherapy of hepatoma with a monoclonal antibody against murine endoglin

Guang-Hong Tan, Feng-Ying Huang, Hua Wang, Yong-Hao Huang, Ying-Ying Lin, Yue-Nan Li

Guang-Hong Tan, Feng-Ying Huang, Hua Wang, Yong-Hao Huang, Ying-Ying Lin, Yue-Nan Li, Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571101, Hainan Province, China

Guang-Hong Tan, Feng-Ying Huang, Pediatric Department of Affiliated Hospital, Hainan Medical College, Haikou 570102, Hainan Province, China

Author contributions: All authors contributed equally to the work.

Supported by the National Natural Science Foundation of China, No. 30360115 and 30560048; the Program for New Century Excellent Talents in University of China, No. NCET-05-0757; the Foundation Project for Natural Science by the Education Department of Hainan Province of China, No. Hjkj200422

Correspondence to: Dr. Guang-Hong Tan, Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571101, Hainan Province, China. tanhoho@163.com

Telephone: +86-898-66893335 Fax: +86-898-66893335

Received: March 1, 2007
Revised: March 5, 2007
Accepted: March 28, 2007
Published online: May 7, 2007

Abstract

AIM: To explore the capability of a monoclonal antibody (mAb) against murine endoglin to inhibit tumor angiogenesis and suppression of hepatoma growth in murine models.

METHODS: A monoclonal antibody against murine endoglin was purified by affinity chromatography and passively transfused through tail veins in two murine hepatoma models. Tumor volume and survival time were observed at three-day intervals for 48 d. Microvessels in tumor tissues were detected by immunohistochemistry against CD31, and angiogenesis in vivo was determined by alginate encapsulated assay. In addition, tumor cell apoptosis was detected by TUNEL assay.

RESULTS: Passive immunotherapy with anti-endoglin mAb could effectively suppress tumor growth, and prolonged the survival time of hepatoma-bearing mice. Angiogenesis was apparently inhibited within the tumor tissues, and the vascularization of alginate beads was also reduced in the mice passively transfused with anti-endoglin mAb. In addition, increased apoptotic cells were observed within the tumor tissues from the mice passively transfused with anti-endoglin mAb.

CONCLUSION: Passive immunotherapy with anti-endoglin mAb effectively inhibits tumor growth via inhibiting tumor angiogenesis and increasing tumor cell apoptosis, which may be highly correlated with the blockage of endoglin-related signal pathway induced by anti-endoglin mAb.

Keywords: Monoclonal antibody, Endoglin, Passive immunotherapy, Tumor angiogenesis, Apoptosis