Inhibitory effect of vascular endothelial growth factors-targeted small interfering RNA on proliferation of gastric cancer cells

doi:10.3748/wjg.v13.i14.2044

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. Apr 14, 2007; 13(14): 2044-2047
Published online Apr 14, 2007. doi: 10.3748/wjg.v13.i14.2044

Inhibitory effect of vascular endothelial growth factors-targeted small interfering RNA on proliferation of gastric cancer cells

Wen-Hua Xu, Yin-Lin Ge, Quan Li, Xiao Zhang, Jian-Hua Duan

Wen-Hua Xu, Yin-Lin Ge, Quan Li, Xiao Zhang, Jian-Hua Duan, Department of Biochemistry and Molecular Biology, Medical College, Qingdao University, Qingdao 266021, Shandong Province, China

Author contributions: All authors contributed equally to the work.

Supported by Science Project of Qingdao Science and Technology Administration, No. 03-1-YN-14

Correspondence to: Professor Yin-Lin Ge, Department of Biochemistry and Molecular Biology, Medical College, Qingdao University, Qingdao 266021, Shandong Province, China. geyinlin@126.com

Telephone: +86-532-83780075

Received: November 16, 2006
Revised: February 15, 2007
Accepted: February 25, 2007
Published online: April 14, 2007

Abstract

AIM: To examine the effects of vascular endothelial growth factor (VEGF)-targeted small interfering RNA (siRNA) on proliferation of gastric cancer cells in vitro.

METHODS: Several siRNAs were transfected into human gastric cancer cell line SGC-7901 with Lipofectamine 2000. Cells not transfected with Lipofectamine^TM 2000 or scrambled (SCR) siRNA served as controls. The inhibitory effect of siRNA on the expression of VEGF mRNA and protein was detected by RT-PCR and ELISA. MTT assay was used to examine the inhibition rate of cell growth. The change in cell cycling of siRNA-treated cells was detected by flow cytometry.

RESULTS: siRNA targeting human VEGF effectively inhibited the proliferation of gastric cancer cell line SGC-7901 and the distribution of cell cycle. The percentage of G₀/G₁ phase was significantly higher in siRNA₁- and siRNA₂-transfected cells than in control cells. The expression of VEGF mRNA was significantly inhibited in siRNA₁- and siRNA₂-transfected cells compared with that in control cells. VEGF protein notably decreased in siRNA-transfected cells, but had no effect on SCR siRNA.

CONCLUSION: VEGF siRNA inhibits proliferation of gastric cancer cells in vitro.

Keywords: siRNA; SGC-7901; Vascular endothelial growth factors; Gene therapy; RNA interference