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World J Gastroenterol. Mar 7, 2006; 12(9): 1468-1471
Published online Mar 7, 2006. doi: 10.3748/wjg.v12.i9.1468
Expression of pituitary adenylate cyclase-activating polypeptide 1 and 2 receptor mRNA in gallbladder tissue of patients with gallstone or gallbladder polyps
Zhen-Hai Zhang, Shuo-Dong Wu, Hong Gao, Gang Shi, Jun-Zhe Jin, Jing Kong, Zhong Tian, Yang Su
Zhen-Hai Zhang, Shuo-Dong Wu, Hong Gao, Gang Shi, Jun-Zhe Jin, Jing Kong, Zhong Tian, Yang Su, No.2 Department of General Surgery, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Hong Gao, Key Laboratory of Congenital Malformation of Public Health Ministry, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Correspondence to: Dr. Shuo-Dong Wu, No.2 Department of General Surgery , Second Affiliated Hospital, China Medical University, Shenyang, 110004, Liaoning Province, China. wushuodong@hotmail.com
Telephone: +86-24-83955062
Received: August 2, 2005
Revised: August 15, 2005
Accepted: August 25, 2005
Published online: March 7, 2006
Abstract

AIM: To detect the expression of pituitary adenylate cyclase-activating polypeptide receptor 1 (VPCAP1-R) and VPCAP2-R mRNA in gallbladder tissues of patients with gallstone or gallbladder polyps.

METHODS: The expression of VPCAP1-R and VPCAP2-R mRNA in gallbladder tissues was detected in 25 patients with gallstone, 8 patients with gallbladder polyps and 7 donors of liver transplantation by reverse transcription polymerase chain reaction (RT-PCR).

RESULTS: The VPCAP2-R mRNA expression level in the control group (1.09±0.58) was lower than that in the gallbladder polyp group (1.64 ± 0.56) and the gallstone group (1.55±0.45) (P < 0.05) while the VPCAP1-R mRNA expression level in the control group (1.15 ± 0.23) was not apparently different from that in the gallbladder polyp group (1.28±0.56) and the gallstone group (1.27 ± 0.38).

CONCLUSION: The abnormal expression of VPCAP2-R mRNA in gallbladder tissue may play a role in the formation of gallbladder stone and gallbladder polyps.

Keywords: VPCAP1-R; VPCAP2-R; RT-PCR; Gallbladder disease