Effects of retrorsine on mouse hepatocyte proliferation after liver injury

doi:10.3748/wjg.v12.i9.1439

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 12, Issue 9

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (3378)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-5) series.

Item

Count

PDF

343

HTML

2731

Figures (1-5)

304

Sum=3378

Mar 7, 2006 (publication date) through Nov 4, 2024

Times Cited of This Article

Times Cited (10)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Rapid Communication

World J Gastroenterol. Mar 7, 2006; 12(9): 1439-1442
Published online Mar 7, 2006. doi: 10.3748/wjg.v12.i9.1439

Effects of retrorsine on mouse hepatocyte proliferation after liver injury

Xiao-Fei Zhou, Qian Wang, Jian-Xin Chu, Ai-Lian Liu

Xiao-Fei Zhou, Qian Wang, Jian-Xin Chu, Ai-Lian Liu, Center for Developmental Biology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China

Supported by the Major State Basic Research Development Program of China, No.001CB509904; National High Technology Research and Development Program of China, 2004AA205010; Shanghai Science & Technology Commission and Shanghai Municipal Education Commission

Correspondence to: Professor Ai-Lian Liu, Center for Developmental Biology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, 1665 Kong Jiang Road, Shanghai 200092, China. al_liu@yahoo.com

Telephone: +86-21-63852733 Fax: +86-21-63852733

Received: March 28, 2005
Revised: May 1, 2005
Accepted: October 10, 2005
Published online: March 7, 2006

Abstract

AIM: To study the effect of retrorsine on mouse he-patocyte proliferation.

METHODS: Mice and rats were treated respectively with two injections of retrorsine (as retrosine-treated group) or saline (as non-treated group) at 2 wk intervals. They received a single injection of carbon tetrachloride (CCl₄) 4 wk later. On d 0, 1, 2, 3, 4, 6, 15 after CCl₄ administration, the animals were killed and their livers were excised. Hematoxylin and eosin (HE) staining and Ki-67 antibody immunohistochemical analysis of liver samples were used to evaluate the pathological changes and hepatocyte proliferation.

RESULTS: In rats treated with retrorsine and CCl₄, the liver displayed obvious megalocytosis, proliferation of mild bile duct, small hepatocyte-forming nodule, which were not found in liver samples from non-treated group. However, in mice treated with retrorsine combined with CCl_4, the liver displayed hepatocyte degeneration and necrosis in perivenous areas. There was no obvious difference between retrorsine-treated group and non-treated group. Ki-67 immunohistochemical analysis showed that in rats treated with retrorsine, the positive hepatocytes mainly found in small hepatocyte nodules, were obviously less than those in non-treated group. The mice treated with retrorsine showed that the number of Ki-67 positive hepatocytes was very high and more than that in non-treated group.

CONCLUSION: Retrorsine has no effect on mouse hepatocyte proliferation.

Keywords: Retrorsine; Mouse; Rat; Hepatocyte proliferation