Effects of retrorsine on mouse hepatocyte proliferation after liver injury

doi:10.3748/wjg.v12.i9.1439

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Mar 7, 2006 (publication date) through Jul 22, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 7, 2006; 12(9): 1439-1442
Published online Mar 7, 2006. doi: 10.3748/wjg.v12.i9.1439

Effects of retrorsine on mouse hepatocyte proliferation after liver injury

Xiao-Fei Zhou, Qian Wang, Jian-Xin Chu, Ai-Lian Liu

Xiao-Fei Zhou, Qian Wang, Jian-Xin Chu, Ai-Lian Liu, Center for Developmental Biology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China

Supported by the Major State Basic Research Development Program of China, No.001CB509904; National High Technology Research and Development Program of China, 2004AA205010; Shanghai Science & Technology Commission and Shanghai Municipal Education Commission

Correspondence to: Professor Ai-Lian Liu, Center for Developmental Biology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, 1665 Kong Jiang Road, Shanghai 200092, China. al_liu@yahoo.com

Telephone: +86-21-63852733 Fax: +86-21-63852733

Received: March 28, 2005
Revised: May 1, 2005
Accepted: October 10, 2005
Published online: March 7, 2006

Abstract

AIM: To study the effect of retrorsine on mouse he-patocyte proliferation.

METHODS: Mice and rats were treated respectively with two injections of retrorsine (as retrosine-treated group) or saline (as non-treated group) at 2 wk intervals. They received a single injection of carbon tetrachloride (CCl₄) 4 wk later. On d 0, 1, 2, 3, 4, 6, 15 after CCl₄ administration, the animals were killed and their livers were excised. Hematoxylin and eosin (HE) staining and Ki-67 antibody immunohistochemical analysis of liver samples were used to evaluate the pathological changes and hepatocyte proliferation.

RESULTS: In rats treated with retrorsine and CCl₄, the liver displayed obvious megalocytosis, proliferation of mild bile duct, small hepatocyte-forming nodule, which were not found in liver samples from non-treated group. However, in mice treated with retrorsine combined with CCl_4, the liver displayed hepatocyte degeneration and necrosis in perivenous areas. There was no obvious difference between retrorsine-treated group and non-treated group. Ki-67 immunohistochemical analysis showed that in rats treated with retrorsine, the positive hepatocytes mainly found in small hepatocyte nodules, were obviously less than those in non-treated group. The mice treated with retrorsine showed that the number of Ki-67 positive hepatocytes was very high and more than that in non-treated group.

CONCLUSION: Retrorsine has no effect on mouse hepatocyte proliferation.

Keywords: Retrorsine, Mouse, Rat, Hepatocyte proliferation