Viral Hepatitis
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Feb 28, 2006; 12(8): 1205-1210
Published online Feb 28, 2006. doi: 10.3748/wjg.v12.i8.1205
Autoimmune thrombocytopenia in response to splenectomy in cirrhotic patients with accompanying hepatitis C
Tetsuro Sekiguchi, Takeaki Nagamine, Hitoshi Takagi, Masatomo Mori
Tetsuro Sekiguchi, Hitoshi Takagi, Masatomo Mori, Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi, Japan
Takeaki Nagamine, School of Health Sciences, Gunma University Graduate School of Medicine, Maebashi, Japan
Author contributions: All authors contributed equally to the work.
Correspondence to: Takeaki Nagamine, MD, School of Health Science, Faculty of Medicine, Gunma University Graduate School of Medicine, Maebashi 371-8514, Japan. mine@health.gunma-u.ac.jp
Telephone: +81-27-2208923 Fax: +81-27-2208923
Received: September 27, 2005
Revised: October 21, 2005
Accepted: October 26, 2005
Published online: February 28, 2006
Abstract

AIM: To estimate the contribution of autoimmune thrombocytopenia to hepatitis C virus-related liver cirrhosis (type C cirrhosis), we evaluated the influence of splenectomy upon platelet-associated immunoglobulin G (PAIgG) levels and platelet numbers.

METHODS: PAIgG titers and immune markers were determined in 24 type C cirrhotic patients with an intact spleen, 17 type C cirrhotic patients submitted to splenectomy, and 21 non-C cirrhosis with an intact spleen.

RESULTS: Thrombocytopenia (PLT<15×104/μL) in type C cirrhosis was diagnosed in all patients with an intact spleen, 8 patients submitted to splenectomy, and in 19 non-C cirrhosis with intact spleen. Elevated titers of PAIgG at more than 25.0 ng/107cells were detected in all cirrhotic patients except for one splenectomized patient. PAIgG titers (ng/107cells) were significantly higher in the type C cirrhosis with an intact spleen (247.9 ± 197.0) compared with the splenectomized patients (125.6±87.8) or non-C cirrhosis (152.4 ± 127.4). PAIgG titers were negatively correlated with platelet counts in type C cirrhotic patients with an intact spleen. In comparison with the type C cirrhosis with an intact spleen, the splenectomized patients had a reduced CD4/CD8 ratio and serum neopterin levels. The spleen index (cm2) was negatively correlated with platelet counts in the non-C cirrhosis, but not in the type C cirrhosis.

CONCLUSION: Our data indicate that the autoimmune mechanism plays an important role in thrombocytosis complicated by HCV-positive cirrhosis. In addition, splenectomy may impair T cells function through, at least in part, a reduction of CD4/CD8 ratio, consequently suppressing PAIgG production.

Keywords: Platelet-associated immunoglobulin G; Autoimmune thrombocytopenia; Liver cirrhosis; Hepatitis C virus; Splenectomy; CD4/CD8 ratio