Evaluation of the role of H pylori infection in pathogenesis of gastric cancer by immunoblot assay

doi:10.3748/wjg.v12.i43.7029

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 12, Issue 43

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (2482)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-1) series, Tables (1-2) series.

Item

Count

PDF

252

HTML

1852

Figures (1-1)

193

Tables (1-2)

185

Sum=2482

Nov 21, 2006 (publication date) through Nov 5, 2024

Times Cited of This Article

Times Cited (7)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Rapid Communication

World J Gastroenterol. Nov 21, 2006; 12(43): 7029-7032
Published online Nov 21, 2006. doi: 10.3748/wjg.v12.i43.7029

Evaluation of the role of H pylori infection in pathogenesis of gastric cancer by immunoblot assay

Kuo-Ching Yang, Alexander Chu, Chao-Sheng Liao, Yu-Min Lin, Gen-Min Wang

Kuo-Ching Yang, Alexander Chu, Chao-Sheng Liao, Yu-Min Lin, Gen-Min Wang, Division of Gastroenterology, Department of Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, China

Kuo-Ching Yang, Taipei Medical University, Taipei, Taiwan, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Alexander Chu, Division of Gastroenterology, Department of Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, No. 95 Wen Chang Road, Shih Lin District, Taipei, Taiwan, China. alex.kec@msa.hinet.net

Telephone: +886-2-28332211 Fax: +886-2-28389335

Received: July 23, 2006
Revised: August 16, 2006
Accepted: August 29, 2006
Published online: November 21, 2006

Abstract

AIM: To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer.

METHODS: A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy: normal control group (25 patients) and gastric cancer group (29 patients). Both groups were further divided into H pylori (+) and H pylori (-) subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique (HelicoBlot 2.0, Genelabs Diagnostics, Singapore).

RESULTS: The positive rate of the immunoblotting test was as high as 88.9% in the H pylori (-) gastric cancer group and only 14.3% in the H pylori (-) normal control group with a statistically significant difference.

CONCLUSION: The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.

Keywords: Western blot; Immunoblotting; Gastric cancer; H pylori; Enzyme-linked immunosorbent assay