Basic Research
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Oct 21, 2006; 12(39): 6325-6330
Published online Oct 21, 2006. doi: 10.3748/wjg.v12.i39.6325
Impact of lipoprotein lipase gene polymorphisms on ulcerative colitis
Toshihito Kosaka, Junji Yoshino, Kazuo Inui, Takao Wakabayashi, Kazumu Okushima, Takashi Kobayashi, Hironao Miyoshi, Yuta Nakamura, Shigekazu Hayashi, Taizou Shiraishi, Masatoshi Watanabe, Takayuki Yamamoto, Ai Nakahara, Takahiko Katoh
Toshihito Kosaka, Junji Yoshino, Kazuo Inui, Takao Wakabayashi, Kazumu Okushima, Takashi Kobayashi, Hironao Miyoshi, Yuta Nakamura, Department of Internal Medicine, Second Teaching Hospital, Fujita Health University School of Medicine, 3-6-10 Otobashi, Nakagawaku, Nagoya 454-8509, Japan
Shigekazu Hayashi, Department of Gastroenterology, Nagoya Ekisaikai Hospital, 4-66 Shounencho, Nakagawaku, Nagoya 454-8502, Japan
Taizou Shiraishi, Department of Pathologic Oncology, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan
Masatoshi Watanabe, School of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan
Takayuki Yamamoto, Inflammatory bowel disease centre and department of surgery, Yokkaichi Social Insulance Hospital, 10-8 Hazuyamacho, Yokkaichi, Mie, Japan
Ai Nakahara, Takahiko Katoh, Department of Public Health, Miyazaki Medical College, University of Miyazaki, 5200 Kihara, Miyazaki 889-1692, Japan
Correspondence to: Dr. Toshihito Kosaka, Department of Internal Medicine, Second Teaching Hospital Fujita Health University School of Medicine, 3-6-10 Otobashi, Nakagawaku, Nagoya 454-8509, Japan. bantaneshoukaki@mbn.nifty.com
Telephone: +81-52-3218171 Fax: +81-52-3239886
Received: July 13, 2006
Revised: July 28, 2006
Accepted: July 29, 2006
Published online: October 21, 2006
Abstract

AIM: To examine the influence of lipoprotein lipase (LPL) gene polymorphism in ulcerative colitis (UC) patients.

METHODS: Peripheral blood was obtained from 131 patients with UC and 106 healthy controls for DNA extraction. We determined LPL gene polymorphisms affecting the enzyme at Ser447stop, as well as HindIII and PvuII polymorphisms using PCR techniques. PCR products were characterized by PCR-RFLP and direct sequencing. Polymorphisms were examined for association with clinical features in UC patients. Genotype frequencies for LPL polymorphisms were also compared between UC patients and controls.

RESULTS: In patients with onset at age 20 years or younger, C/G and G/G genotypes for Ser447stop polymorphism were more prevalent than C/C genotype (OR = 3.13, 95% CI = 0.95-10.33). Patients with H+/- or H-/- genotype for HindIII polymorphism also were more numerous than those with H+/+ genotype (OR = 2.51, 95% CI = 0.85-7.45). In the group with H+/+ genotype for HindIII polymorphism, more patients had serum triglyceride concentrations over 150 mg/dL than patients with H+/- or H-/- genotype (P < 0.01, OR = 6.46, 95% CI = 1.39-30.12). Hypertriglycemia was also more prevalent in patients with P+/+ genotypes for PvuII polymorphism (P < 0.05, OR = 3.0, 95% CI = 1.06-8.50). Genotype frequency for LPL polymorphism did not differ significantly between UC patients and controls.

CONCLUSION: Ser447stop and HindIII LPL polymorphisms may influence age of onset of UC, while HindIII and PvuII polymorphisms influence serum triglyceride in UC patients.

Keywords: Ulcerative colitis; Lipoprotein lipase; Lipid metabolism; Triglyceride