Differentiation of rat bone marrow stem cells in liver after partial hepatectomy

doi:10.3748/wjg.v12.i31.5051

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 21, 2006; 12(31): 5051-5054
Published online Aug 21, 2006. doi: 10.3748/wjg.v12.i31.5051

Differentiation of rat bone marrow stem cells in liver after partial hepatectomy

Yu-Tao Zhan, Yu Wang, Lai Wei, Bin Liu, Hong-Song Chen, Xu Cong, Ran Fei

Yu-Tao Zhan, Bin Liu, Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Yu Wang, Lai Wei, Hong-Song Chen, Xu Cong, Ran Fei, Hepatology Institute, Beijing University People’s Hospital, Beijing 100044, China

Co-first-author: Yu Wang

Supported by the National High Technology Research And Development Program of China (863 Program), No. 2001AA217151

Correspondence to: Yu-Tao Zhan, Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China. yutaozhan@263.net

Telephone: +86-10-58268473

Received: September 23, 2005
Revised: October 15, 2005
Accepted: October 26, 2005
Published online: August 21, 2006

Abstract

AIM: To investigate the differentiation of rat bone marrow stem cells in liver after partial hepatectomy.

METHODS: Bone marrow cells were collected from the tibia of rat with partial hepatectomy, the medial and left hepatic lobes were excised. The bone marrow stem cells (Thy⁺CD3^-CD45RA^- cells) were enriched from the bone marrow cells by depleting red cells and fluorescence-activated cell sorting. The sorted bone marrow stem cells were labeled by PKH26-GL in vitro and autotransplanted by portal vein injection. After 2 wk, the transplanted bone marrow stem cells in liver were examined by the immunohistochemistry of albumin (hepatocyte-specific marker).

RESULTS: The bone marrow stem cells (Thy⁺CD3^-CD45RA^- cells) accounted for 2.8% of bone marrow cells without red cells. The labeling rate of 10 μM PKH26-GL on sorted bone marrow stem cells was about 95%. There were sporadic PKH26-GL-labeled cells among hepatocytes in liver tissue section, and some of the cells expressed albumin.

CONCLUSION: Rat bone marrow stem cells can differentiate into hepatocytes in regenerative environment and may participate in liver regeneration after partial hepatectomy.

Keywords: Bone marrow stem cells; Liver regeneration; Differentiation